Description
| Product Name: | Human RAN Recombinant Protein |
| Product Code: | RPPB4397 |
| Size: | 10µg |
| Species: | Human |
| Target: | RAN |
| Synonyms: | ARA24, Gsp1, TC4, GTP-binding nuclear protein Ran, GTPase Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24, Ras-like, RANP1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | RAN Human solution containing 20mM Tris-HCl pH-8,1mM DTT & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL |
RAN is a GTP-binding protein that participates in nucleocytoplasmic transport. RAN is necessary for the import of protein into the nucleus and also for RNA export. RAN takes part in chromatin condensation and control of cell cycle. The RAN complex with BIRC5 participates in mitotic spindle formation by being as a physical scaffold to assist the delivery of the RAN effector molecule TPX2 to microtubules. RUN increases AR-mediated transactivation.
RAN Recombinant�Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (1-216 a.a.) and having a molecular mass of 26.5 kDa. The RAN is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RAN: a small GTPase belonging to the RAS superfamily that regulates the nucleo-cytoplasmic traffic through the nuclear pore complex, the condensation of chromatin, spindle assembly and cytokinesis, as well as the organization of non-centrosomal microtubules. A major regulator of mitosis and a potential therapeutic target in cancers expressing higher Ras/MEK/ERK and PI3K/Akt/mTORC1 activities. Required for the import of protein into and RNA export from the nucleus. Cycles between the nucleus and cytoplasm in this process. The Ran guanine nucleotide-exchange factor (RanGEF) is nuclear, promoting the GTP-bound state, while the Ran GTPase activating protein (RanGAP) is cytoplasmic, promoting GDP-bound state. RanGDP is imported into the nucleus by nuclear transport factor-2 (NUTF2), where RanGEF catalyses nucleotide exchange and generates RanGTP. RanGTP then binds to transport factors, such as KPNB1 and CAS, and is exported to the cytoplasm, where RanGAP stimulates GTP hydrolysis. Regulates microtubule polymerization during mitosis; micritubule localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1), a Ran-GEF. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Regulates the formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. GTP-Ran interacts with survivin at S and M phases of the cell cycle. Ran-survivin complexes play a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Interacts with TERT following the tyrosine phosphorylation of TERT induced by H2O2, inducing nuclear export of TERT. Interacts with MAD2L2. Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cell cycle regulation; Nuclear receptor co-regulator; G protein, monomeric, Ran; G protein, monomeric; Nuclear export; G protein Chromosomal Location of Human Ortholog: 12q24.3 Cellular Component: centriole; chromatin; cytoplasm; cytosol; membrane; midbody; nucleolus; nucleoplasm; nucleus; recycling endosome Molecular Function:chromatin binding; GDP binding; GTP binding; GTPase activity; protein binding Biological Process: DNA metabolic process; mitosis; mitotic spindle organization and biogenesis; positive regulation of protein binding; pre-microRNA export from nucleus; protein export from nucleus; protein import into nucleus, translocation; ribosomal large subunit export from nucleus; ribosomal small subunit export from nucleus; RNA-mediated gene silencing; signal transduction; tRNA export from nucleus; viral reproduction |
| NCBI Summary: | RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62826 |
| NCBI GenInfo Identifier: | 51338598 |
| NCBI Gene ID: | 5901 |
| NCBI Accession: | P62826.3 |
| UniProt Secondary Accession: | P62826,P17080, P28746, P28747, Q6IPB2, Q86V08, Q8NI90 Q9CSP3, Q9CWI7, Q9CZA2, Q9UDJ5, A8K3Z8, |
| UniProt Related Accession: | P62826,AAB24940 |
| Molecular Weight: | 24,423 Da |
| NCBI Full Name: | GTP-binding nuclear protein Ran |
| NCBI Synonym Full Names: | RAN, member RAS oncogene family |
| NCBI Official Symbol: | RAN�� |
| NCBI Official Synonym Symbols: | TC4; Gsp1; ARA24�� |
| NCBI Protein Information: | GTP-binding nuclear protein Ran |
| UniProt Protein Name: | GTP-binding nuclear protein Ran |
| UniProt Synonym Protein Names: | Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein |
| Protein Family: | Ranakinin |
| UniProt Gene Name: | RAN�� |
| UniProt Entry Name: | RAN_HUMAN |
