Description
| Product Name: | Human RALB Recombinant Protein |
| Product Code: | RPPB4393 |
| Size: | 10µg |
| Species: | Human |
| Target: | RALB |
| Synonyms: | Ras-related protein Ral-B, RALB. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The RALB solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAANKS KGQSSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLLVFSIT EHESFTATAE FREQILRVKA EEDKIPLLVV GNKSDLEERR QVPVEEARSK AEEWGVQYVE TSAKTRANVD KVFFDLMREI RTKKMSENKD KNGKKSSKNK KSFKERC |
Ras-related protein Ral-B (RALB) is a member of a subfamily of Ras-related GTPases. RALB is activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. RALB has a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. In addition, RALB is required for suppression of apoptosis.
RALB Human Recombinant fused with a 24 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 227 amino acids (1-203 a.a.) and having a molecular mass of 25.6kDa. The RALB is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RALB: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis. Interacts with EXOC2 and EXOC8. Interacts with RALBP1 via its effector domain. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Ras; G protein; G protein, monomeric Chromosomal Location of Human Ortholog: 2q14.2 Cellular Component: plasma membrane; exocyst; autophagic vacuole; midbody Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; ubiquitin protein ligase binding; ATPase binding Biological Process: regulation of exocyst localization; positive regulation of protein binding; nerve growth factor receptor signaling pathway; apoptosis; metabolic process; regulation of exocyst assembly; Ras protein signal transduction; cytokinesis; positive regulation of protein amino acid phosphorylation; negative regulation of protein binding; signal transduction; cellular response to starvation |
| NCBI Summary: | This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P11234 |
| NCBI GenInfo Identifier: | 131835 |
| NCBI Gene ID: | 5899 |
| NCBI Accession: | P11234.1 |
| UniProt Secondary Accession: | P11234,Q53T32, Q6ZS74, B4E040, |
| UniProt Related Accession: | P11234 |
| Molecular Weight: | 206 |
| NCBI Full Name: | Ras-related protein Ral-B |
| NCBI Synonym Full Names: | v-ral simian leukemia viral oncogene homolog B |
| NCBI Official Symbol: | RALB�� |
| NCBI Protein Information: | ras-related protein Ral-B; RAS-like protein B; ras related GTP binding protein B; v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein) |
| UniProt Protein Name: | Ras-related protein Ral-B |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RALB�� |
| UniProt Entry Name: | RALB_HUMAN |
