Description
| Product Name: | Human RAB32 Recombinant Protein |
| Product Code: | RPPB4360 |
| Size: | 20µg |
| Species: | Human |
| Target: | RAB32 |
| Synonyms: | RAB32 member RAS oncogene family, ras-related protein Rab-32. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The RAB32 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 5mM DTT, 0.2M NaCl, 0.2M EDTA and 50% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAGGGA GDPGLGAAAA PAPETREHLF KVLVIGELGV GKTSIIKRYV HQLFSQHYRA TIGVDFALKV LNWDSRTLVR LQLWDIAGQE RFGNMTRVYY KEAVGAFVVF DISRSSTFEA VLKWKSDLDS KVHLPNGSPI PAVLLANKCD QNKDSSQSPS QVDQFCKEHG FAGWFETSAK DNINIEEAAR FLVEKILVNH QSFPNEENDV DKIKLDQETL RAENKSQCC |
RAB32 is a member of the small GTPase superfamily. RAB32 controls ER calcium management and disturbs the specific enhancement of calnexin on the MAM (mitochondria-associated membrane), however avoids affecting the ER distribution of protein-disulfide isomerase and mitofusin-2. Furthermore, RAB32 regulates the targeting of PKA (cAMP-dependent protein kinase) to mitochondrial and ER membranes and by inactivation or overexpression enhances the phosphorylation of Drp1 and Bad. Using a combination of its functions as a regulator of MAM properties and a PKA-anchoring protein, the activity and expression level of RAB32 define the speed of apoptosis onset.
RAB32 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 249 amino acids (1-225) and having a molecular mass of 27.6 kDa.RAB32 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RAB32: Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission. Belongs to the small GTPase superfamily. Rab family. |
| UniProt Protein Details: | Protein type:G protein, monomeric; G protein; G protein, monomeric, Rab Chromosomal Location of Human Ortholog: 6q24.3 Cellular Component: early endosome; melanosome; membrane; mitochondrion; phagocytic vesicle; trans-Golgi network Molecular Function:GTP-dependent protein binding; GTPase activity; protein binding Biological Process: antigen processing and presentation |
| NCBI Summary: | The protein encoded by this gene anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. The encoded protein also appears to be involved in autophagy and melanosome secretion. Variations in this gene may be linked to leprosy. [provided by RefSeq, Dec 2015] |
| UniProt Code: | Q13637 |
| NCBI GenInfo Identifier: | 2833245 |
| NCBI Gene ID: | 10981 |
| NCBI Accession: | Q13637.3 |
| UniProt Related Accession: | Q13637 |
| Molecular Weight: | 24,997 Da |
| NCBI Full Name: | Ras-related protein Rab-32 |
| NCBI Synonym Full Names: | RAB32, member RAS oncogene family |
| NCBI Official Symbol: | RAB32�� |
| NCBI Protein Information: | ras-related protein Rab-32 |
| UniProt Protein Name: | Ras-related protein Rab-32 |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAB32�� |
| UniProt Entry Name: | RAB32_HUMAN |
