Description
| Product Name: | Human RAB13 Recombinant Protein |
| Product Code: | RPPB4345 |
| Size: | 10µg |
| Species: | Human |
| Target: | RAB13 |
| Synonyms: | RAB13 member RAS oncogene family, Cell growth-inhibiting gene 4 protein, Growth-inhibiting gene 4 protein, RAS-associated protein RAB13. |
| Source: | Escherichia Coli |
| Physical Appearance: | RAB13 is supplied as a sterile filtered clear solution. |
| Formulation: | RAB13 protein (0.25mg/ml) is supplied in 20mM Tris-HCL, pH-8, 1mM DTT, 200mM NaCl, 1mM EDTA and 20% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNNTYIST IGIDFKIRTV DIEGKKIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ NWMKSIKENA SAGVERLLLG NKCDMEAKRK VQKEQADKLA REHGIRFFET SAKSSMNVDE AFSSLARDIL LKSGGRRSGN GNKPPSTDLK TCDKKNTNKC |
RAB13 is a member of the small GTPase superfamily which are nontransforming monomeric GTP-binding proteins that hold 4 extremely conserved regions that take part in GTP binding and hydrolysis. In addition, RAB proteins have a vital role in the endocytic pathways. The GTPase Rab13 regulates the assembly of functional epithelial tight junctions (TJs) and is expressed in every phase of preimplantation development.
RAB13 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 220 amino acids (1-200) and having a molecular mass of 24.7 kDa.The RAB13 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RAB13: Could participate in polarized transport, in the assembly and/or the activity of tight junctions. Belongs to the small GTPase superfamily. Rab family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Rab; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 1q21.2 Cellular Component: cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; endocytic vesicle; Golgi apparatus; lamellipodium; lateral plasma membrane; neuron projection; plasma membrane; recycling endosome; tight junction; trans-Golgi network Molecular Function:GTP binding; GTPase activity; protein binding Biological Process: cellular response to insulin stimulus; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; endosome transport; neurite development; protein secretion; vesicle docking during exocytosis |
| NCBI Summary: | This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12. [provided by RefSeq, Jan 2013] |
| UniProt Code: | P51153 |
| NCBI GenInfo Identifier: | 1710016 |
| NCBI Gene ID: | 5872 |
| NCBI Accession: | P51153.1 |
| UniProt Secondary Accession: | P51153,Q5U0A6, Q6GPG6, Q96GU4, A8K6B5, D3DV67, |
| UniProt Related Accession: | P51153 |
| Molecular Weight: | 22,774 Da |
| NCBI Full Name: | Ras-related protein Rab-13 |
| NCBI Synonym Full Names: | RAB13, member RAS oncogene family |
| NCBI Official Symbol: | RAB13�� |
| NCBI Official Synonym Symbols: | GIG4�� |
| NCBI Protein Information: | ras-related protein Rab-13 |
| UniProt Protein Name: | Ras-related protein Rab-13 |
| UniProt Synonym Protein Names: | Cell growth-inhibiting gene 4 protein |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAB13�� |
| UniProt Entry Name: | RAB13_HUMAN |
