Description
| Product Name: | Human PTEN Recombinant Protein |
| Product Code: | RPPB4331 |
| Size: | 5µg |
| Species: | Human |
| Target: | PTEN |
| Synonyms: | Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, EC 3.1.3.67, EC 3.1.3.16, EC 3.1.3.48, Phosphatase and tensin homolog, Mutated in multiple advanced cancers 1, PTEN, MMAC1, TEP1, BZS, MHAM, PTEN1, 10q23del, MGC11227. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PTEN solution contains 20mM Tris-HCl buffer (pH 8.0), 1mM EDTA, 2mM DTT, 100mM NaCl, and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MTAIIKEIVS RNKRRYQEDG FDLDLTYIYP NIIAMGFPAE RLEGVYRNNI DDVVRFLDSK HKNHYKIYNL CAERHYDTAK FNCRVAQYPF EDHNPPQLEL IKPFCEDLDQ WLSEDDNHVA AIHCKAGKGR TGVMICAYLL HRGKFLKAQE ALDFYGEVRT RDKKGVTIPS QRRYVYYYSY LLKNHLDYRP VALLFHKMMF ETIPMFSGGT CNPQFVVCQL KVKIYSSNSG PTRREDKFMY FEFPQPLPVC GDIKVEFFHK QNKMLKKDKM FHFWVNTFFI PGPEETSEKV ENGSLCDQEI DSICSIERAD NDKEYLVLTL TKNDLDKANK DKANRYFSPN FKVKLYFTKT VEEPSNPEAS SSTSVTPDVS DNEPDHYRYS DTTDSDPENE PFDEDQHTQI TKV |
PTEN, a tumor suppressor, has been implicated in a large number of human tumors and is conserved from humans to worms. PTEN has a tensin like domain and a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Characterization of PTEN protein showed that it is a phosphatase that acts on proteins and on 3-phosphorylated phosphoinositides, and can therefore modulate signal transduction pathways that involve lipid second messengers. In contrast to most of the protein tyrosine phosphatases, PTEN preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and acts as a tumor suppressor by negative regulation of AKT/PKB signaling pathway. Recent results indicate that at least part of its role is to regulate the activity of the serine/threonine kinase AKT/PKB, and thus influence cell survival signaling.
PTEN Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 423 amino acids (1- 403 a.a.) and having a molecular mass of 49.3kDa.The PTEN is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PTEN: a phosphoinositide 3-phosphatase (PIP3) and a tumor suppressor implicated in a wide variety of human cancers. Dephosphorylates inositol phospholipids generated by the activation of the phosphoinositide 3 kinase (PI3K). A major negative regulator of the PI3K/Akt signaling pathway. Possesses a C-terminal regulatory domain that contains three phosphorylation sites which may regulate its stability. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - inositol phosphate; EC 3.1.3.48; Phosphatase, lipid; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; EC 3.1.3.16; EC 3.1.3.67; Tumor suppressor Chromosomal Location of Human Ortholog: 10q23.3 Cellular Component: nucleoplasm; postsynaptic membrane; PML body; internal side of plasma membrane; neuron projection; cell projection; mitochondrion; cytoplasm; plasma membrane; extracellular region; dendritic spine; cytosol; nucleus Molecular Function:phosphatidylinositol-3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; platelet-derived growth factor receptor binding; magnesium ion binding; protein tyrosine phosphatase activity; protein serine/threonine phosphatase activity; protein kinase binding; PDZ domain binding; protein binding; enzyme binding; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; phosphoprotein phosphatase activity; lipid binding Biological Process: central nervous system development; male mating behavior; nerve growth factor receptor signaling pathway; prepulse inhibition; response to arsenic; regulation of cellular component size; heart development; anaphase-promoting complex activation during mitotic cell cycle; Wnt receptor signaling pathway through beta-catenin; T cell receptor signaling pathway; regulation of B cell apoptosis; myelin maintenance in the central nervous system; response to glucose stimulus; negative regulation of axonogenesis; response to drug; rhythmic synaptic transmission; fibroblast growth factor receptor signaling pathway; dentate gyrus development; negative regulation of myelination; social behavior; forebrain morphogenesis; negative regulation of organ growth; response to ethanol; response to zinc ion; inositol phosphate dephosphorylation; phosphatidylinositol biosynthetic process; endothelial cell migration; positive regulation of transcription factor activity; multicellular organismal response to stress; negative regulation of apoptosis; negative regulation of phagocytosis; synapse maturation; inositol phosphate metabolic process; apoptosis; regulation of protein stability; locomotory behavior; negative regulation of cell size; platelet-derived growth factor receptor signaling pathway; protein amino acid dephosphorylation; regulation of myeloid cell apoptosis; response to estradiol stimulus; negative regulation of cell proliferation; locomotor rhythm; learning and/or memory; synaptogenesis; negative regulation of protein amino acid phosphorylation; phospholipid metabolic process; positive regulation of cell proliferation; brain morphogenesis; protein kinase B signaling cascade; nerve-nerve synaptic transmission; angiogenesis; negative regulation of cell migration; response to nutrient; negative regulation of epithelial cell proliferation; aging; cardiac muscle development; epidermal growth factor receptor signaling pathway; cell migration; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; protein stabilization; central nervous system neuron axonogenesis; maternal behavior; negative regulation of focal adhesion formation; response to ATP; memory; cell proliferation; G1/S-specific negative regulation of cyclin-dependent protein kinase activity; innate immune response; phosphoinositide dephosphorylation; regulation of cyclin-dependent protein kinase activity; negative regulation of phosphoinositide 3-kinase cascade Disease: Meningioma, Familial, Susceptibility To; Thyroid Carcinoma, Follicular; Melanoma, Cutaneous Malignant, Susceptibility To, 1; Bannayan-riley-ruvalcaba Syndrome; Cowden Syndrome 1; Squamous Cell Carcinoma, Head And Neck; Prostate Cancer; Glioma Susceptibility 2; Vacterl Association With Hydrocephalus; Endometrial Cancer; Macrocephaly/autism Syndrome |
| NCBI Summary: | This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P60484 |
| NCBI GenInfo Identifier: | 42560209 |
| NCBI Gene ID: | 5728 |
| NCBI Accession: | P60484.1 |
| UniProt Secondary Accession: | P60484,O00633, O02679, Q6ICT7, B2R904, F2YHV0, |
| UniProt Related Accession: | P60484 |
| Molecular Weight: | 19,796 Da |
| NCBI Full Name: | Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN |
| NCBI Synonym Full Names: | phosphatase and tensin homolog |
| NCBI Official Symbol: | PTEN�� |
| NCBI Official Synonym Symbols: | BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del�� |
| NCBI Protein Information: | phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; mitochondrial PTENalpha; phosphatase and tensin-like protein; mutated in multiple advanced cancers 1; mitochondrial phosphatase and tensin protein alpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN |
| UniProt Protein Name: | Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN |
| UniProt Synonym Protein Names: | Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog |
| Protein Family: | Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase |
| UniProt Gene Name: | PTEN�� |
| UniProt Entry Name: | PTEN_HUMAN |
