Description
| Product Name: | Human PSMA3 Recombinant Protein |
| Product Code: | RPPB2198 |
| Size: | 20µg |
| Species: | Human |
| Target: | PSMA3 |
| Synonyms: | proteasome (prosome, macropain) subunit alpha type-3, HC8, Macropain subunit C8, Multicatalytic endopeptidase complex subunit C8, Proteasome component C8, PSC3, PSC8, EC 3.4.25.1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PSMA3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSSIGTGYDL SASTFSPDGR VFQVEYAMKA VENSSTAIGI RCKDGVVFGV EKLVLSKLYE EGSNKRLFNV DRHVGMAVAG LLADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG YWGCAIGKAR QAAKTEIEKL QMKEMTCRDI VKEVAKIIYI VHDEVKDKAF ELELSWVGEL TNGRHEIVPK DIREEAEKYA KESLKEEDES DDDNM |
The proteasome is a multicatalytic proteinase complex which holds an extremely organized 20S core structure shaped like a ring. The central structure is assembled from 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. There is a high concentration of proteasomes all over the eukaryotic cells and their job is to cleave peptides in an ATP/ubiquitin-dependent procedure in a non-lysosomal pathway. PSMA3 encodes several of the peptidase T1A family which is a 20S core alpha subunit. Two alternative transcripts encoding diverse isoforms were recognized.
PSMA3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 275 amino acids (1-255) and having a molecular mass of 30.6 kDa.PSMA3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PSMA3: a proteasomal protein of the T1A peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Two alternatively spliced isoforms have been identified. |
| UniProt Protein Details: | Protein type:Proteasome complex; Protease; EC 3.4.25.1 Chromosomal Location of Human Ortholog: 14q23 Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; cytosol; nucleus Molecular Function:threonine endopeptidase activity; protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P25788 |
| NCBI GenInfo Identifier: | 130859 |
| NCBI Gene ID: | 5684 |
| NCBI Accession: | P25788.2 |
| UniProt Secondary Accession: | P25788,Q86U83, Q8N1D8, Q9BS70, B2RCK6, |
| UniProt Related Accession: | P25788 |
| Molecular Weight: | Calculated MW: 27kDa/28kDaObserved MW: 28kDa |
| NCBI Full Name: | Proteasome subunit alpha type-3 |
| NCBI Synonym Full Names: | proteasome (prosome, macropain) subunit, alpha type, 3 |
| NCBI Official Symbol: | PSMA3�� |
| NCBI Official Synonym Symbols: | HC8; PSC3�� |
| NCBI Protein Information: | proteasome subunit alpha type-3; macropain subunit C8; proteasome subunit C8; proteasome component C8; multicatalytic endopeptidase complex subunit C8 |
| UniProt Protein Name: | Proteasome subunit alpha type-3 |
| UniProt Synonym Protein Names: | Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome component C8 |
| Protein Family: | Phenol-soluble modulin alpha 3 peptide |
| UniProt Gene Name: | PSMA3�� |
| UniProt Entry Name: | PSA3_HUMAN |
