Description
| Product Name: | Human PPP1R8 Recombinant Protein |
| Product Code: | RPPB2168 |
| Size: | 10µg |
| Species: | Human |
| Target: | PPP1R8 |
| Synonyms: | Protein phosphatase 1 regulatory subunit 8, nuclear inhibitor of protein phosphatase-1 alpha, Protein phosphatase 1 regulatory inhibitor subunit 8, ARD1, NIPP1, PRO2047, ARD-1, activator of RNA decay, nuclear subunit of PP-1, RNase E. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PPP1R8 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 2mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAAAANSGSS LPLFDCPTWA GKPPPGLHLD VVKGDKLIEK LIIDEKKYYL FGRNPDLCDF TIDHQSCSRV HAALVYHKHL KRVFLIDLNS THGTFLGHIR LEPHKPQQIP IDSTVSFGAS TRAYTLREKP QTLPSAVKGD EKMGGEDDEL KGLLGLPEEE TELDNLTEFN TAHNKRISTL TIEEGNLDIQ RPKRKRKNSR VTFSEDDEII NPEDVDPSVG RFRNMVQTAV VPVKKKRVEG PGSLGLEESG SRRMQNFAFS GGLYGGLPPT HSEAGSQPHG IHGTALIGGL PMPYPNLAPD VDLTPVVPSA VNMNPAPNPA VYNPEAVNEP KKKKYAKEAW PGKKPTPSLL IVEHHHHHH |
Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8) is the RNA-binding subunit of a key species of protein phosphatase-1 in the nucleus. The PPP1R8 enzyme is an inhibitor subunit of the main nuclear protein phosphatase-1 (PP-1). PPP1R8 has RNA-binding activity however it doesn�t cleave RNA and may target PP-1 to RNA-associated substrates. Two isoforms of the PPP1R8 protein are specific inhibitors of type 1 serine/threonine protein phosphatases which can bind but not cleave RNA. The third isoform of the PPP1R8 protein is deficient in of the phosphatase inhibitory activity nonetheless it is a single-strand endoribonuclease comparable to RNase E of E. coli.
PPP1R8 Human Recombinant produced in E. coli is a single polypeptide chain containing 359 amino acids (1-351) and having a molecular mass of 39.5 kDa.PPP1R8 is fused to a 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Inhibitor; Protein phosphatase, regulatory subunit; Ribonuclease; EC 3.1.4.-; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p35.3 Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck; nucleus Molecular Function:protein binding; ribonuclease E activity; DNA binding; protein phosphatase type 1 regulator activity; RNA binding; endonuclease activity; type 1 serine/threonine specific protein phosphatase inhibitor activity Biological Process: cell proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; RNA catabolic process; negative regulation of catalytic activity; mRNA processing |
| NCBI Summary: | This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12972 |
| NCBI GenInfo Identifier: | 19863082 |
| NCBI Gene ID: | 5511 |
| NCBI Accession: | Q12972.2 |
| UniProt Secondary Accession: | Q12972,Q5TEJ2, Q5TEJ4, Q5TIF2, Q6PKF6, Q9UBH1, Q9UBZ0 |
| UniProt Related Accession: | Q12972 |
| Molecular Weight: | 351 |
| NCBI Full Name: | Nuclear inhibitor of protein phosphatase 1 |
| NCBI Synonym Full Names: | protein phosphatase 1, regulatory subunit 8 |
| NCBI Official Symbol: | PPP1R8�� |
| NCBI Official Synonym Symbols: | ARD1; ARD-1; NIPP1; NIPP-1; PRO2047�� |
| NCBI Protein Information: | nuclear inhibitor of protein phosphatase 1; RNase E; activator of RNA decay; nuclear subunit of PP-1; protein phosphatase 1 regulatory subunit 8; nuclear inhibitor of protein phosphatase-1 alpha; protein phosphatase 1, regulatory (inhibitor) subunit 8 |
| UniProt Protein Name: | Nuclear inhibitor of protein phosphatase 1 |
| UniProt Synonym Protein Names: | Protein phosphatase 1 regulatory inhibitor subunit 8Including the following 1 domains:Activator of RNA decay (EC:3.1.4.-)Alternative name(s):ARD-1 |
| Protein Family: | Nuclear inhibitor of protein phosphatase |
| UniProt Gene Name: | PPP1R8�� |
| UniProt Entry Name: | PP1R8_HUMAN |
