Description
| Product Name: | Human PPIL2 Recombinant Protein |
| Product Code: | RPPB2155 |
| Size: | 10µg |
| Species: | Human |
| Target: | PPIL2 |
| Synonyms: | CYC4, Cyp-60, CYP60, hCyP-60, Peptidyl-prolyl cis-trans isomerase-like 2, PPIase, Rotamase PPIL2, Cyclophilin-60, Cyclophilin-like protein Cyp-60, PPIL2, MGC787, FLJ39930, MGC33174. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear colorless solution. |
| Formulation: | The PPIL2 solution contains 20mM Tris-HCl pH-8, 0.1M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLIK LNFSKNSEGK YHCPVLFTVF TNNTHIVAVR TTGNVYAYEA VEQLNIKAKN FRDLLTDEPF SRQDIITLQD PTNLDKFNVS NFYHVKNNMK IIDPDEEKAK QDPSYYLKNT NAETRETLQE YKEFKGDEI LAATMKAPEK KKVDKLNAAH YSTGKVSASF TSTAMVPETT EAAAIDEDV LRYQFVKKKG YVRLHTNKGD LNLELHCDLT PKTCENFIRL CKKHYYDGTI FHRSIRNFVI QGGDPTGTGT GGESYWGKPF KDEFRPNLSH TGRGILSMAN SGPNSNRSQF FITFRSCAYL DKKHTIFGRV VGGFDVLTAM ENVESDPKTD RPKEEIRIDA TTVFVDPYEE ADAQIAQERK TQLKVAPETK VKSSQPQAGS QGPQTFRQGV GKYINPAATE QQRKSPQPVP LSPCPRRSPV GVLGTSAPGS SRLPDDH |
| Biological Activity: | Specific activity is > 290 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. |
PPIL2 is part of the cyclophilin family of peptidylprolyl isomerases which are highly conserved ubiquitous proteins that play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. PPIL2 interacts with the proteinase inhibitor eglin c and is localized in the nucleus. PPIL2 increases folding of proteins andcatalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
PPIL2 Human Recombinant fused to 20 amino acid His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 547 amino acids (1-527 a.a.) and having a molecular mass of 61.6 kDa. The PPIL2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPIL2: a peptidylprolyl isomerase (PPIase) of the cyclophilin-type family. PPIases accelerate the folding of proteins by catalyzing the cis-trans isomerization of proline imidic peptide bonds. Two alternatively-spliced isoforms have been described.Protein type: Isomerase; Ubiquitin ligase; Spliceosome; Ligase; Cyclophilin; EC 5.2.1.8; Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 22q11.21Cellular Component: cytoplasm; Golgi lumen; nucleoplasm; nucleus; plasma membraneMolecular Function: peptidyl-prolyl cis-trans isomerase activity; protein bindingBiological Process: leukocyte migration; protein polyubiquitination |
| UniProt Protein Details: | |
| NCBI Summary: | This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015] |
| UniProt Code: | Q13356 |
| NCBI GenInfo Identifier: | 23813917 |
| NCBI Gene ID: | 23759 |
| NCBI Accession: | Q13356.1 |
| UniProt Secondary Accession: | Q13356,Q13357, Q8TAH2, Q9BWR8 |
| UniProt Related Accession: | Q13356 |
| Molecular Weight: | 59,458 Da |
| NCBI Full Name: | Peptidyl-prolyl cis-trans isomerase-like 2 |
| NCBI Synonym Full Names: | peptidylprolyl isomerase like 2 |
| NCBI Official Symbol: | PPIL2�� |
| NCBI Official Synonym Symbols: | CYC4; CYP60; UBOX7; Cyp-60; hCyP-60�� |
| NCBI Protein Information: | peptidyl-prolyl cis-trans isomerase-like 2 |
| UniProt Protein Name: | Peptidyl-prolyl cis-trans isomerase-like 2 |
| UniProt Synonym Protein Names: | CYC4 |
| Protein Family: | |
| UniProt Gene Name: | PPIL2�� |
| UniProt Entry Name: | PPIL2_HUMAN |
