Description
| Product Name: | Human PNPT1 Recombinant Protein |
| Product Code: | RPPB2147 |
| Size: | 10µg |
| Species: | Human |
| Target: | PNPT1 |
| Synonyms: | Polyribonucleotide Nucleotidyltransferase 1, PolynucleotidePhosphorylase-Like Protein, Polynucleotide Phosphorylase 1, 3-5 RNA ExonucleaseOLD35, PNPase Old-35, EC 2.7.7.8, PNPase 1, COXPD13, DFNB70, PNPASE, OLD35, PolyribonucleotideNucleotidyltransferase 1, Mitochondrial, Deafness, Autosomal Recessive 70, PolynucleotidePhosphorylase, 3-5 RNA Exonuclease, EC 2.7.7, Old-35, Polyribonucleotidenucleotidyltransferase 1, mitochondrial, 3'-5' RNA exonuclease OLD35, PNPaseold-35. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | PNPT1 protein solution (0.25mg/ml) containing Phosphatebuffered saline (pH7.4), 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSAVAVDLG NRKLEISSGK LARFADGSAV VQSGDTAVMV TAVSKTKPSPSQFMPLVVDY RQKAAAAGRI PTNYLRREIG TSDKEILTSR IIDRSIRPLF PAGYFYDTQV LCNLLAVDGVNEPDVLAING ASVALSLSDI PWNGPVGAVR IGIIDGEYVV NPTRKEMSSS TLNLVVAGAP KSQIVMLEASAENILQQDFC HAIKVGVKYT QQIIQGIQQL VKETGVTKRT PQKLFTPSPE IVKYTHKLAM ERLYAVFTDYEHDKVSRDEA VNKIRLDTEE QLKEKFPEAD PYEIIESFNV VAKEVFRSIV LNEYKRCDGR DLTSLRNVSCEVDMFKTLHG SALFQRGQTQ VLCTVTFDSL ESGIKSDQVI TAINGIKDKN FMLHYEFPPY ATNEIGKVTGLNRRELGHGA LAEKALYPVI PRDFPFTIRV TSEVLESNGS SSMASACGGS LALMDSGVPI SSAVAGVAIGLVTKTDPEKG EIEDYRLLTD ILGIEDYNGD MDFKIAGTNK GITALQADIK LPGIPIKIVM EAIQQASVAKKEILQIMNKT ISKPRASRKE NGPVVETVQV PLSKRAKFVG PGGYNLKKLQ AETGVTISQV DEETFSVFAPTPSAMHEARD FITEICKDDQ EQQLEFGAVY TATITEIRDT GVMVKLYPNM TAVLLHNTQL DQRKIKHPTALGLEVGQEIQ VKYFGRDPAD GRMRLSRKVL QSPATTVVRT LNDRSSIVMG EPISQSSSNS Q |
Polyribonucleotide nucleotidyltransferase 1, also known as PNPT1 ispredominantly localized in the mitochondrial intermembrane space and is implicated inthe import of RNA to mitochondria. Mutations in PNPT1 have been connected withcombined oxidative phosphorylation deficiency-13 as well as autosomal recessivenonsyndromic deafness-70. Related pseudogenes have been found on chromosomes 3 & 7.
PNPT1 Human Recombinant produced in E.Coli is a single, non-glycosylatedpolypeptide chain containing 761 amino acids (46-783 a.a) and having amolecular mass of 83.3kDa.PNPT1 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PNPT1: RNA-binding protein implicated in numerous RNA metabolic processes. Hydrolyzes single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c- myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. Belongs to the polyribonucleotide nucleotidyltransferase family. |
| UniProt Protein Details: | Protein type:Mitochondrial; Transferase; RNA-binding; EC 2.7.7.8; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine Chromosomal Location of Human Ortholog: 2p15 Cellular Component: cytoplasm; mitochondrial intermembrane space; mitochondrion Molecular Function:3'-5'-exoribonuclease activity; miRNA binding; poly(rG) binding; poly(U) binding; polyribonucleotide nucleotidyltransferase activity; protein binding Biological Process: mRNA catabolic process; negative regulation of growth; protein homooligomerization; regulation of cellular respiration; RNA catabolic process; RNA polyadenylation Disease: Combined Oxidative Phosphorylation Deficiency 13; Deafness, Autosomal Recessive 70 |
| NCBI Summary: | The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012] |
| UniProt Code: | Q8TCS8 |
| NCBI GenInfo Identifier: | 115502437 |
| NCBI Gene ID: | 87178 |
| NCBI Accession: | Q8TCS8.2 |
| UniProt Secondary Accession: | Q8TCS8,Q53SU0, Q68CN1, Q7Z7D1, Q8IWX1, Q96T05, Q9BRU3 Q9BVX0, |
| UniProt Related Accession: | Q8TCS8 |
| Molecular Weight: | 85,951 Da |
| NCBI Full Name: | Polyribonucleotide nucleotidyltransferase 1, mitochondrial |
| NCBI Synonym Full Names: | polyribonucleotide nucleotidyltransferase 1 |
| NCBI Official Symbol: | PNPT1�� |
| NCBI Official Synonym Symbols: | OLD35; DFNB70; PNPASE; old-35; COXPD13�� |
| NCBI Protein Information: | polyribonucleotide nucleotidyltransferase 1, mitochondrial |
| UniProt Protein Name: | Polyribonucleotide nucleotidyltransferase 1, mitochondrial |
| UniProt Synonym Protein Names: | 3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; PNPase 1; Polynucleotide phosphorylase-like protein |
| UniProt Gene Name: | PNPT1�� |
| UniProt Entry Name: | PNPT1_HUMAN |
