Description
| Product Name: | Human PFDN6 Recombinant Protein |
| Product Code: | RPPB4232 |
| Size: | 10µg |
| Species: | Human |
| Target: | PFDN6 |
| Synonyms: | Prefoldin Subunit 6, PFD6, H2-KE2, KE-2, HKE2, HLA class II region expressed gene KE2, MGC70744. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The PFDN6 protein solution (0.5mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAELIQKKLQ GEVEKYQQLQ KDLSKSMSGR QKLEAQLTEN NIVKEELALL DGSNVVFKLL GPVLVKQELG EARATVGKRL DYITAEIKRY ESQLRDLERQ SEQQRETLAQ LQQEFQRAQA AKAGAPGKA |
PFDN6 is a subunit of the heteromeric prefoldin complex that chaperones developing actin and alpha- and beta-tubulin chains until they are transferred to the cytosolic chaperonin containing TCP1 (CCT) complex. PFDN6 binds specifically to cytosolic chaperonin (c-CPN), transfers target proteins to it and bind to developing polypeptide chain to promote folding in a setting where there are many competing pathways for nonnative proteins.
PFDN6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 149 amino acids (1-129a.a.) and having a molecular mass of 16.7 kDa. PFDN6 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PFDN6: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit beta family. |
| UniProt Protein Details: | Protein type:Chaperone; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 6p21.32 Cellular Component: cytoplasm; prefoldin complex Molecular Function:chaperone binding Biological Process: chaperone-mediated protein complex assembly; protein folding |
| NCBI Summary: | PFDN6 is a subunit of the heteromeric prefoldin complex that chaperones nascent actin (see MIM 102560) and alpha- and beta-tubulin (see MIM 602529 and MIM 191130, respectively) chains pending their transfer to the cytosolic chaperonin containing TCP1 (MIM 186980) (CCT) complex (Hansen et al., 1999 [PubMed 10209023]).[supplied by OMIM, Jul 2010] |
| UniProt Code: | O15212 |
| NCBI GenInfo Identifier: | 6016432 |
| NCBI Gene ID: | 10471 |
| NCBI Accession: | O15212.1 |
| UniProt Related Accession: | O15212 |
| Molecular Weight: | Calculated: 14kDaObserved: 14kDa |
| NCBI Full Name: | Prefoldin subunit 6 |
| NCBI Synonym Full Names: | prefoldin subunit 6 |
| NCBI Official Symbol: | PFDN6�� |
| NCBI Official Synonym Symbols: | HKE2; KE-2; PFD6; H2-KE2�� |
| NCBI Protein Information: | prefoldin subunit 6 |
| UniProt Protein Name: | Prefoldin subunit 6 |
| UniProt Synonym Protein Names: | Protein Ke2 |
| Protein Family: | Prefoldin |
| UniProt Gene Name: | PFDN6�� |
