Description
| Product Name: | Human PDIA3 Recombinant Protein |
| Product Code: | RPPB2083 |
| Size: | 10µg |
| Species: | Human |
| Target: | PDIA3 |
| Synonyms: | ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC, ER60, Protein disulfide-isomerase A3, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 60, ER protein 60, 58 kDa microsomal protein, Endoplasmic reticulum resident protein 57, ER protein 57, 58 kDa glucose-regulated protein, PDIA3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The PDIA3 protein solution contains 20mM Tris-HCl, pH-8, 1mM DTT, 0.1M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL |
| Biological Activity: | Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT. |
PDIA3 is an enzyme that belongs to the endoplasmic reticulum and interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. PDIA3 has protein disulfide isomerase activity. Complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. PDIA3 is expressed in the lumbar spinal cord from rats submitted to peripheral lesion during neonatal period. PDIA3 interacts with thiazide-sensitive sodium-chloride cotransporter in the kidney and is induced by glucose deprivation. PDIA3 is part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is important for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface.
PDIA3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 518 amino acids (25-505 a.a.) and having a molecular wieght of 58.5 kDa. The PDIA3 is fused to 37 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway. |
| UniProt Protein Details: | Protein type:Chaperone; Endoplasmic reticulum; Protease; Isomerase; EC 5.3.4.1; Apoptosis; Phospholipase Chromosomal Location of Human Ortholog: 15q15 Cellular Component: smooth endoplasmic reticulum; cell surface; focal adhesion; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome; nucleus Molecular Function:disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; protein import into nucleus; cell redox homeostasis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein amino acid N-linked glycosylation via asparagine; signal transduction; proteolysis; post-translational protein modification; protein retention in ER |
| NCBI Summary: | This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30101 |
| NCBI GenInfo Identifier: | 2507461 |
| NCBI Gene ID: | 2923 |
| NCBI Accession: | P30101.4 |
| UniProt Secondary Accession: | P30101,Q13453, Q14255, Q8IYF8, Q9UMU7, |
| UniProt Related Accession: | P30101,AAB37397 |
| Molecular Weight: | 56,782 Da |
| NCBI Full Name: | Protein disulfide-isomerase A3 |
| NCBI Synonym Full Names: | protein disulfide isomerase family A, member 3 |
| NCBI Official Symbol: | PDIA3�� |
| NCBI Official Synonym Symbols: | P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n�� |
| NCBI Protein Information: | protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n |
| UniProt Protein Name: | Protein disulfide-isomerase A3 |
| UniProt Synonym Protein Names: | 58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60 |
| Protein Family: | Protein disulfide-isomerase |
| UniProt Gene Name: | PDIA3�� |
| UniProt Entry Name: | PDIA3_HUMAN |
