Description
| Product Name: | Human OTUB2 Recombinant Protein |
| Product Code: | RPPB4175 |
| Size: | 25µg |
| Species: | Human |
| Target: | OTUB2 |
| Synonyms: | Ubiquitin thioesterase OTUB2, Deubiquitinating enzyme OTUB2, OTU domain-containing ubiquitin aldehyde-binding protein 2, Otubain-2, Ubiquitin-specific-processing protease OTUB2, OTUB2, C14orf137, OTB2, OTU2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | OTUB2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 50mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSETSFNLIS EKCDILSILR DHPENRIYRR KIEELSKRFT AIRKTKGDGN CFYRALGYSY LESLLGKSRE IFKFKERVLQ TPNDLLAAGF EEHKFRNFFN AFYSVVELVE KDGSVSSLLK VFNDQSASDH IVQFLRLLTS AFIRNRADFF RHFIDEEMDI KDFCTHEVEP MATECDHIQI TALSQALSIA LQVEYVDEMD TALNHHVFPE AATPSVYLLY KTSHYNILYA ADKH |
Ubiquitin thioesterase OTUB2 (OTUB2) is a member of the peptidase C65 family. OTUB2 functions as a hydrolase which can remove conjugated ubiquitin from proteins in vitro and may thus play a key regulatory role at the level of protein turnover by preventing degradation.
OTUB2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 254 amino acids (1-234 a.a.) and having a molecular mass of 29.4kDa.OTUB2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | OTUB2: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C65 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.4.19.12; Ubiquitin-specific protease; Hydrolase Chromosomal Location of Human Ortholog: 14q32.12 Cellular Component: nucleus Molecular Function:NEDD8-specific protease activity; protein binding; ubiquitin binding; ubiquitin-specific protease activity Biological Process: protein deubiquitination |
| NCBI Summary: | This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q96DC9 |
| NCBI GenInfo Identifier: | 44888285 |
| NCBI Gene ID: | 78990 |
| NCBI Accession: | Q96DC9.2 |
| UniProt Secondary Accession: | Q96DC9,Q6IA10, Q9H6T1, |
| UniProt Related Accession: | Q96DC9 |
| Molecular Weight: | 8,637 Da |
| NCBI Full Name: | Ubiquitin thioesterase OTUB2 |
| NCBI Synonym Full Names: | OTU deubiquitinase, ubiquitin aldehyde binding 2 |
| NCBI Official Symbol: | OTUB2�� |
| NCBI Official Synonym Symbols: | OTB2; OTU2; C14orf137�� |
| NCBI Protein Information: | ubiquitin thioesterase OTUB2 |
| UniProt Protein Name: | Ubiquitin thioesterase OTUB2 |
| UniProt Synonym Protein Names: | Deubiquitinating enzyme OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2; Otubain-2; Ubiquitin-specific-processing protease OTUB2 |
| Protein Family: | Ubiquitin thioesterase |
| UniProt Gene Name: | OTUB2�� |
| UniProt Entry Name: | OTUB2_HUMAN |
