Description
| Product Name: | Human NUCB2 Recombinant Protein |
| Product Code: | RPPB4136 |
| Size: | 10µg |
| Species: | Human |
| Target: | NUCB2 |
| Synonyms: | Nucleobindin-2, DNA-binding protein NEFA, Gastric cancer antigen Zg4, NUCB2, NEFA, Nesfatin. |
| Source: | Escherichia Coli |
| Formulation: | NUCB2 (Nesfatin) was filtered (0.4 �m) and lyophilized�from 0.5 mg/ml in 20mM Tris and 50mM NaCl, pH 7.5. |
| Solubility: | It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Amino Acid Sequence: | MKHHHHHHAS VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL |
Nucleobindin-2 (also known as NUCB2 or Nesfatin) is a EF-hand calcium-binding protein. Nucleobindin-2 takes part in calcium homeostasis and is a multifunctional protein that interacts with Ca(2+) nucleic acids & various regulatory proteins in different signaling pathways. NUCB2 (Nesfatin)�is localized in neuronal perikarya and dendrites of mouse brain.
The Recombinant Human NUCB2 (Nesfatin)�produced in E.coli has a molecular mass of 10.79kDa containing�92 amino acid residues of the human NUCB2 and fused to a�10 a.a. His tag at N-terminus.
| UniProt Protein Function: | NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 11p15.1 Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; nuclear envelope; cytosol Molecular Function:protein binding; DNA binding; calcium ion binding |
| NCBI Summary: | This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P80303 |
| NCBI GenInfo Identifier: | 224471846 |
| NCBI Gene ID: | 4925 |
| NCBI Accession: | P80303.2 |
| UniProt Secondary Accession: | P80303,Q8NFT5, A8K642, D3DQX5, |
| UniProt Related Accession: | P80303 |
| Molecular Weight: | 50,201 Da |
| NCBI Full Name: | Nucleobindin-2 |
| NCBI Synonym Full Names: | nucleobindin 2 |
| NCBI Official Symbol: | NUCB2�� |
| NCBI Official Synonym Symbols: | NEFA; HEL-S-109�� |
| NCBI Protein Information: | nucleobindin-2; nesfatin 1; nesfatin-1; prepronefastin; prepronesfatin; nucleobinding 2; DNA-binding protein NEFA; gastric cancer antigen Zg4; epididymis secretory protein Li 109 |
| UniProt Protein Name: | Nucleobindin-2 |
| UniProt Synonym Protein Names: | DNA-binding protein NEFA; Gastric cancer antigen Zg4; PrepronesfatinCleaved into the following chain:Nesfatin-1 |
| Protein Family: | Nucleobindin |
| UniProt Gene Name: | NUCB2�� |
| UniProt Entry Name: | NUCB2_HUMAN |
