Description
| Product Name: | Human NRAS Recombinant Protein |
| Product Code: | RPPB4121 |
| Size: | 10µg |
| Species: | Human |
| Target: | NRAS |
| Synonyms: | GTPase NRas, HRAS1, ALPS4, N-ras, NRAS1, NS6, Transforming protein N-Ras. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The NRAS protein solution contains 20mM Tris-HCl pH-7.5, 100mM NaCl, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ GVEDAFYTLV REIRQYRMKK LNSSDDGTQG CMGLPC |
NRAS binds GDP/GTP and has intrinsic GTPase activity. NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This transport is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. NRAS is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this NRAS gene result in juvenile myelomonocytic leukemia.
NRAS Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-186 a.a.) and having a molecular mass of 20.8kDa. The NRAS is purified by conventional chromatography.
| UniProt Protein Function: | NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric; G protein; G protein, monomeric, Ras Chromosomal Location of Human Ortholog: 1p13.2 Cellular Component: Golgi apparatus; membrane; plasma membrane Molecular Function:protein complex binding Biological Process: axon guidance; epidermal growth factor receptor signaling pathway; leukocyte migration; MAPKKK cascade; Ras protein signal transduction; stimulatory C-type lectin receptor signaling pathway Disease: Colorectal Cancer; Melanocytic Nevus Syndrome, Congenital; Melanosis, Neurocutaneous; Nevus, Epidermal; Noonan Syndrome 6; Ras-associated Autoimmune Leukoproliferative Disorder; Schimmelpenning-feuerstein-mims Syndrome; Thyroid Carcinoma, Follicular |
| NCBI Summary: | This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011] |
| UniProt Code: | P01111 |
| NCBI GenInfo Identifier: | 131883 |
| NCBI Gene ID: | 4893 |
| NCBI Accession: | P01111.1 |
| UniProt Secondary Accession: | P01111,Q14971, Q15104, Q15282, |
| UniProt Related Accession: | P01111 |
| Molecular Weight: | 21,229 Da |
| NCBI Full Name: | GTPase NRas |
| NCBI Synonym Full Names: | neuroblastoma RAS viral oncogene homolog |
| NCBI Official Symbol: | NRAS�� |
| NCBI Official Synonym Symbols: | NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1�� |
| NCBI Protein Information: | GTPase NRas |
| UniProt Protein Name: | GTPase NRas |
| UniProt Synonym Protein Names: | Transforming protein N-Ras |
| Protein Family: | GTPase |
| UniProt Gene Name: | NRAS�� |
| UniProt Entry Name: | RASN_HUMAN |
