Description
| Product Name: | Human NKIRAS1 Recombinant Protein |
| Product Code: | RPPB4099 |
| Size: | 5µg |
| Species: | Human |
| Target: | NKIRAS1 |
| Synonyms: | NF-kappa-B inhibitor-interacting Ras-like protein 1, I-kappa-B-interacting Ras-like protein 1, Kappa B-Ras protein 1, KappaB-Ras1, NKIRAS1, KBRAS1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | NKIRAS1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGKGCKVVVC GLLSVGKTAI LEQLLYGNHT IGMEDCETME DVYMASVETD RGVKEQLHLY DTRGLQEGVE LPKHYFSFAD GFVLVYSVNN LESFQRVELL KKEIDKFKDK KEVAIVVLGN KIDLSEQRQV DAEVAQQWAK SEKVRLWEVT VTDRKTLIEP FTLLASKLSQ PQSKSSFPLP GRKNKGNSNS EN |
NF-kappa-B inhibitor-interacting Ras-like protein 1 (NKIRAS1) is a member of the small GTPase superfamily. NKIRAS1 is an unusual Ras-like protein which acts as a potent regulator of NF-kappa-B activity by thwarting the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, describing why NFKBIB is more resistant to degradation. NKIRAS1 functions by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
NKIRAS1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 212 amino acids (1-192 a.a) and having a molecular mass of 23.8kDa (Molecular weight on SDS-PAGE will appear higher).NKIRAS1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NKIRAS1: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Ras; G protein; G protein, monomeric Chromosomal Location of Human Ortholog: 3p24.2 |
| UniProt Code: | Q9NYS0 |
| NCBI GenInfo Identifier: | 74753075 |
| NCBI Gene ID: | 28512 |
| NCBI Accession: | Q9NYS0.1 |
| UniProt Secondary Accession: | Q9NYS0,Q96K18, |
| UniProt Related Accession: | Q9NYS0 |
| Molecular Weight: | 21,643 Da |
| NCBI Full Name: | NF-kappa-B inhibitor-interacting Ras-like protein 1 |
| NCBI Synonym Full Names: | NFKB inhibitor interacting Ras like 1 |
| NCBI Official Symbol: | NKIRAS1�� |
| NCBI Official Synonym Symbols: | KBRAS1; kappaB-Ras1�� |
| NCBI Protein Information: | NF-kappa-B inhibitor-interacting Ras-like protein 1 |
| UniProt Protein Name: | NF-kappa-B inhibitor-interacting Ras-like protein 1 |
| UniProt Synonym Protein Names: | I-kappa-B-interacting Ras-like protein 1; Kappa B-Ras protein 1; KappaB-Ras1 |
| Protein Family: | NF-kappa-B inhibitor-interacting Ras-like protein |
| UniProt Gene Name: | NKIRAS1�� |
| UniProt Entry Name: | KBRS1_HUMAN |
