Human NF-kB p65 (Nuclear Factor Kappa B p65) ELISA Kit
The Human NF-κB p65 (Nuclear Factor Kappa B p65) ELISA Kit is a powerful tool designed for the precise measurement of NF-κB p65 levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a diverse range of research applications.NF-κB p65 is a key transcription factor that plays a critical role in regulating immune responses, inflammation, and cell survival. Dysregulation of NF-κB signaling has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions.
As such, the measurement of NF-κB p65 levels can provide valuable insights into disease pathology and guide the development of targeted therapeutics.With its user-friendly protocol and robust performance, the Human NF-κB p65 ELISA Kit is an indispensable tool for researchers seeking to better understand the molecular mechanisms underlying NF-κB signaling and its implications in health and disease. Trust in this kit to deliver reliable and reproducible data, paving the way for groundbreaking discoveries in the field of biomedical research.
Product Name:
Human NF-kB p65 (Nuclear Factor Kappa B p65) ELISA Kit
SKU:
HUES02431
Target:
Human NF-kB p65 (Nuclear Factor Kappa B p65)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NFKB-p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NFKB-p65. You can calculate the concentration of Human NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
86-96
93-105
Average (%)
98
91
99
1:4
Range (%)
88-103
86-100
83-95
Average (%)
95
91
88
1:8
Range (%)
91-105
81-96
85-97
Average (%)
99
88
92
1:16
Range (%)
88-103
85-97
83-98
Average (%)
95
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
95
EDTA plasma (n=5)
92-105
100
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.49
3.97
0.52
1.39
3.93
Standard deviation
0.03
0.06
0.21
0.03
0.07
0.13
C V (%)
5.56
4.03
5.29
5.77
5.04
3.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NFKB-p65 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NFKB-p65 in samples. No significant cross-reactivity or interference between Human NFKB-p65 and analogues was observed.
