Description
| Product Name: | Human NEIL1 Recombinant Protein |
| Product Code: | RPPB2019 |
| Size: | 5µg |
| Species: | Human |
| Target: | NEIL1 |
| Synonyms: | Endonuclease 8-like 1, DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, Nei homolog 1, NEH1, Nei-like protein 1, NEIL1, NEI1, hFPG1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The NEIL1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl, 1mM DTT and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS |
NEIL1 is a member of a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. NEIL1 participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines.
NEIL1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 410 amino acids (1-390) and having a molecular mass of 45.8kDa.NEIL1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | NEIL1: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Belongs to the FPG family. |
| UniProt Protein Details: | Protein type:DNA repair, damage; Hydrolase; EC 4.2.99.18; Lyase Chromosomal Location of Human Ortholog: 15q24.2 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; hydrolase activity, acting on glycosyl bonds; protein C-terminus binding Biological Process: base-excision repair; depyrimidination; negative regulation of nuclease activity; response to oxidative stress |
| NCBI Summary: | This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q96FI4 |
| NCBI GenInfo Identifier: | 56404654 |
| NCBI Gene ID: | 79661 |
| NCBI Accession: | Q96FI4.3 |
| UniProt Secondary Accession: | Q96FI4,Q6ZRA7, Q86XW7, Q9H6C3, D3DW75, |
| UniProt Related Accession: | Q96FI4 |
| Molecular Weight: | 43,684 Da |
| NCBI Full Name: | Endonuclease 8-like 1 |
| NCBI Synonym Full Names: | nei like DNA glycosylase 1 |
| NCBI Official Symbol: | NEIL1�� |
| NCBI Official Synonym Symbols: | FPG1; NEI1; hFPG1�� |
| NCBI Protein Information: | endonuclease 8-like 1 |
| UniProt Protein Name: | Endonuclease 8-like 1 |
| UniProt Synonym Protein Names: | DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; NEH1; Nei-like protein 1 |
| Protein Family: | Endonuclease |
| UniProt Gene Name: | NEIL1�� |
| UniProt Entry Name: | NEIL1_HUMAN |
