Description
| Product Name: | Human NCEH1 Recombinant Protein |
| Product Code: | RPPB5907 |
| Size: | 20µg |
| Species: | Human |
| Target: | NCEH1 |
| Synonyms: | AADACL1, NCEH, Neutral cholesterol ester hydrolase 1, Arylacetamide deacetylase-like, KIAA1363, NCEH1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | NCEH1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAEELNA VIVSIEYRLV PKVYFPEQIH DVVRATKYFL KPEVLQKYMV DPGRICISGD SAGGNLAAAL GQQFTQDASL KNKLKLQALI YPVLQALDFN TPSYQQNVNT PILPRYVMVK YWVDYFKGNY DFVQAMIVNN HTSLDVEEAA AVRARLNWTS LLPASFTKNY KPVVQTTGNA RIVQELPQLL DARSAPLIAD QAVLQLLPKT YILTCEHDVL RDDGIMYAKR LESAGVEVTL DHFEDGFHGC MIFTSWPTNF SVGIRTRNSY IKWLDQNL |
Neutral cholesterol ester hydrolase 1 (NCEH1), hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. NCEH1 is responsible for cholesterol ester hydrolysis in macrophages, by this means contributing to the development of atherosclerosis. NCEH1 contributes also to cancer pathogenesis by promoting tumor cell migration. NCEH1 is involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds.
NCEH1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (1-275a.a) and having a molecular mass of 33.6kDa. NCEH1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AADACL1: a single-pass type II membrane microsomal protein. Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (acetyl-glyceryl-ether-phosphorylcholine). Elevated in invasive cancer cells from several different tumor types. May be involved in the elevated neutral ether lipid content and elevated LPA signaling in cancer cells. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration. A key enzymatic node regulating an ether lipid signaling network in cancer; regulates MAGE and lysophospholipid levels in invasive cancer cells. Three alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:Hydrolase; Membrane protein, integral; EC 3.1.1.- Chromosomal Location of Human Ortholog: 3q26.31 Cellular Component: membrane; endoplasmic reticulum; integral to membrane Biological Process: lipid catabolic process |
| UniProt Code: | Q6PIU2 |
| NCBI GenInfo Identifier: | 226423952 |
| NCBI Gene ID: | 57552 |
| NCBI Accession: | NP_001139750.1 |
| UniProt Secondary Accession: | Q6PIU2,Q86WZ1, Q9P2I4, B7Z2K4, B7Z3A1, B7Z5U2, B7Z906 B7ZAW6, F5H7K4, |
| UniProt Related Accession: | Q6PIU2 |
| Molecular Weight: | |
| NCBI Full Name: | neutral cholesterol ester hydrolase 1 isoform c |
| NCBI Synonym Full Names: | neutral cholesterol ester hydrolase 1 |
| NCBI Official Symbol: | NCEH1�� |
| NCBI Official Synonym Symbols: | NCEH; AADACL1�� |
| NCBI Protein Information: | neutral cholesterol ester hydrolase 1; arylacetamide deacetylase-like 1 |
| UniProt Protein Name: | Neutral cholesterol ester hydrolase 1 |
| UniProt Synonym Protein Names: | Arylacetamide deacetylase-like 1 |
| Protein Family: | Neutral cholesterol ester hydrolase |
| UniProt Gene Name: | NCEH1�� |
| UniProt Entry Name: | NCEH1_HUMAN |
