Description
| Product Name: | Human MTDH Recombinant Protein |
| Product Code: | RPPB4016 |
| Size: | 10µg |
| Species: | Human |
| Target: | MTDH |
| Synonyms: | Metadherin, Lysine-Rich CEACAM1 Co-Isolated Protein, Astrocyte Elevated Gene-1 Protein, Metastasis Adhesion Protein, Astrocyte Elevated Gene 1, 3D3/LYRIC, AEG-1, LYRIC, AEG1, LYRIC/3D3, 3D3, MTDH. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | MTDH was filtered (0.4�m) and lyophilized in 20mM Tris buffer and 50mM NaCl, pH 7.5. |
| Solubility: | It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. MTDH is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MKHHHHHHASVSSGLNENLT VNGGGWNEKS VKLSSQISAG EEKWNSVSPA SAGKRKAEPS AWSQDTGDAN TNGKDWGRSW SDRSIFSGIG STAEPVSQST TSDYQWDVSR NQPYIDDEWS GLNGLSSADP NSDWNAPAEE WGNWVDEERA SLLKSQEPIP DDQKVSDDDK EKGEGALPTG KSKKKKKKKK KQGEDN |
Metadherin (MTDH) downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. MTDH activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Furthermore, MTDH promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a central component in tumor cell expansion. In addition, MTDH promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Metadherin also induces chemoresistance.
Metadherin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Val271-Asn456) containing 196 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 21.5kDa.
| UniProt Protein Function: | Lyric: a protein of unknown function. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Nucleolus; Cell adhesion Chromosomal Location of Human Ortholog: 8q22.1 Cellular Component: nuclear body; intercellular canaliculus; endoplasmic reticulum membrane; nuclear membrane; tight junction; endoplasmic reticulum; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; nucleolus; integral to membrane; nucleus Molecular Function:NF-kappaB binding; protein binding; double-stranded RNA binding; transcription coactivator activity Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; lipopolysaccharide-mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of autophagy; negative regulation of apoptosis; activation of NF-kappaB transcription factor |
| UniProt Code: | Q86UE4 |
| NCBI GenInfo Identifier: | 28277147 |
| NCBI Gene ID: | 92140 |
| NCBI Accession: | AAH45642.1 |
| UniProt Secondary Accession: | Q86UE4,Q05DH2, Q52QU9, Q6PK07, Q8TCX3, |
| Molecular Weight: | 63,837 Da |
| NCBI Full Name: | Metadherin |
| NCBI Synonym Full Names: | metadherin |
| NCBI Official Symbol: | MTDH�� |
| NCBI Official Synonym Symbols: | 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3�� |
| NCBI Protein Information: | protein LYRIC |
| UniProt Protein Name: | Protein LYRIC |
| UniProt Synonym Protein Names: | 3D3/LYRIC; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein |
| Protein Family: | Protein LYRIC |
| UniProt Gene Name: | MTDH�� |
| UniProt Entry Name: | LYRIC_HUMAN |
