Description
| Product Name: | Human MED20 Recombinant Protein |
| Product Code: | RPPB3950 |
| Size: | 20µg |
| Species: | Human |
| Target: | MED20 |
| Synonyms: | Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP, PRO0213. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | MED20 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.5), 0.2M NaCl, 50% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMH NSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR |
Mediator Complex Subunit 20 (MED20) is a subunit of the Mediator complex, which is a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II, and general initiation factors. The Mediator functions as a link to transmit information from gene-specific regulatory proteins to the basal RNA polymerase II transcription apparatus. Mediator is recruited to promoters by direct interactions with regulatory proteins and functions as a scaffold for the compilation of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
MED20 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 235 amino acids (1-212 a.a.) and having a molecular mass of 25kDa.MED20 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TRFP: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 20 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: nucleoplasm; Srb-mediator complex Molecular Function:protein binding; DNA-directed RNA polymerase activity Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; gene expression |
| NCBI Summary: | MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM, Aug 2009] |
| UniProt Code: | Q9H944 |
| NCBI GenInfo Identifier: | 30023851 |
| NCBI Gene ID: | 9477 |
| NCBI Accession: | NP_004266.2 |
| UniProt Secondary Accession: | Q9H944,O95821, Q5T8J4, Q9Y429, B4DE08, |
| UniProt Related Accession: | Q9H944 |
| Molecular Weight: | 13,540 Da |
| NCBI Full Name: | mediator of RNA polymerase II transcription subunit 20 |
| NCBI Synonym Full Names: | mediator complex subunit 20 |
| NCBI Official Symbol: | MED20�� |
| NCBI Official Synonym Symbols: | TRFP; PRO0213�� |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 20; TRF-proximal protein homolog; Trf (TATA binding protein-related factor)-proximal homolog; hTRFP; mediator complex subunit 20mediator complex subunit 20mediator complex subunit 20Trf (TATA binding protein-related factor)-proximal homolog |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 20 |
| UniProt Synonym Protein Names: | Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP |
| Protein Family: | Putative mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED20�� |
| UniProt Entry Name: | MED20_HUMAN |
