Description
| Product Name: | Human MDH2 Recombinant Protein |
| Product Code: | RPPB1930 |
| Size: | 10µg |
| Species: | Human |
| Target: | MDH2 |
| Synonyms: | M-MDH, MDH, MOR1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MDH2 1mg/ml protein solution contains 20mM Tris-HCl pH-7.5 and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGPEQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAEVFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQLTALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLLLGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK |
| Biological Activity: | Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25�C. |
MDH2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH2 is localized to the mitochondria and takes part in the malate-aspartate shuttle that functions in the metabolic coordination between cytosol and mitochondria. MDH2 is highy expressed in the adrenal system, small intestine, heart and pancreas.
MDH2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids (25-338 a.a.) and having a molecular mass of 35.2 kDa. The MDH2 is fused to a 21 amino acid His tag at N-terminus and purified by conventional chromatography.
| UniProt Protein Function: | MDH2: mitochondrial malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. May play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.1.37; Oxidoreductase Chromosomal Location of Human Ortholog: 7cen-q22 Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane; nucleus Molecular Function:protein self-association; L-malate dehydrogenase activity; malate dehydrogenase (NADP+) activity Biological Process: cellular metabolic process; malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; pathogenesis; cellular carbohydrate metabolic process; internal protein amino acid acetylation; gluconeogenesis |
| NCBI Summary: | Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
| UniProt Code: | P40926 |
| NCBI GenInfo Identifier: | 215274114 |
| NCBI Gene ID: | 4191 |
| NCBI Accession: | P40926.3 |
| UniProt Secondary Accession: | P40926,O43682, A8K414, B2RE78, B4DE44, E9PDB2, |
| UniProt Related Accession: | P40926 |
| Molecular Weight: | 338 |
| NCBI Full Name: | Malate dehydrogenase, mitochondrial |
| NCBI Synonym Full Names: | malate dehydrogenase 2, NAD (mitochondrial) |
| NCBI Official Symbol: | MDH2�� |
| NCBI Official Synonym Symbols: | MDH; MOR1; M-MDH; MGC:3559�� |
| NCBI Protein Information: | malate dehydrogenase, mitochondrial |
| UniProt Protein Name: | Malate dehydrogenase, mitochondrial |
| Protein Family: | Malate dehydrogenase |
| UniProt Gene Name: | MDH2�� |
| UniProt Entry Name: | MDHM_HUMAN |
