Description
| Product Name: | Human MARCKSL1 Recombinant Protein |
| Product Code: | RPPB3932 |
| Size: | 10µg |
| Species: | Human |
| Target: | MARCKSL1 |
| Synonyms: | F52, MACMARCKS, MLP, MLP1, MRP, MARCKS-related protein, MARCKS-like protein 1, Macrophage myristoylated alanine-rich C kinase substrate, MARCKSL1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MARCKSL1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMGSQSSK APRGDVTAEE AAGASPAKAN GQENGHVKSN GDLSPKGEGE SPPVNGTDEA AGATGDAIEP APPSQGAEAK GEVPPKETPK KKKKFSFKKP FKLSGLSFKR NRKEGGGDSS ASSPTEEEQE QGEIGACSDE GTAQEGKAAA TPESQEPQAK GAEASAASEE EAGPQATEPS TPSGPESGPT PASAEQNE |
MARCKS-related protein (MARCKSL1) which is a part of MARCKS family of PKC substrate is broadly used in the signal transduction studies as an indicator of PKC activation. MARCKSL1 plays a part in the coordination of membrane-cytoskeletal signaling events, including secretion, migration, phagocytosis and cell adhesion and is expressed in a variety of tissues with highest levels found in testis and uterus. Furthermore, MARCKSL1 serves as a regulator of Integrin activation and is thought to regulate Integrin-dependent signal transduction pathways, particularly those involved in macrophage spreading.
MARCKSL1 Human Recombinant produced in E. coli is a single polypeptide chain containing 218 amino acids (1-195) and having a molecular mass of 21.9kDa. MARCKSL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MLP: Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration. When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration. May also affect cancer cell migration. May be involved in coupling the protein kinase C and calmodulin signal transduction systems. Belongs to the MARCKS family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 1p35.1 Cellular Component: cytoplasm; plasma membrane Molecular Function:calmodulin binding; actin binding Biological Process: positive regulation of cell proliferation |
| NCBI Summary: | This gene encodes a member of the myristoylated alanine-rich C-kinase substrate (MARCKS) family. Members of this family play a role in cytoskeletal regulation, protein kinase C signaling and calmodulin signaling. The encoded protein affects the formation of adherens junction. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on the long arm of chromosomes 6 and 10. [provided by RefSeq, Jun 2012] |
| UniProt Code: | P49006 |
| NCBI GenInfo Identifier: | 1346576 |
| NCBI Gene ID: | 65108 |
| NCBI Accession: | P49006.2 |
| UniProt Related Accession: | P49006 |
| Molecular Weight: | |
| NCBI Full Name: | MARCKS-related protein |
| NCBI Synonym Full Names: | MARCKS like 1 |
| NCBI Official Symbol: | MARCKSL1�� |
| NCBI Official Synonym Symbols: | F52; MLP; MRP; MLP1; MACMARCKS�� |
| NCBI Protein Information: | MARCKS-related protein |
| UniProt Protein Name: | MARCKS-related protein |
| UniProt Synonym Protein Names: | MARCKS-like protein 1; Macrophage myristoylated alanine-rich C kinase substrate; Mac-MARCKS; MacMARCKS |
| Protein Family: | MIP-related peptides |
| UniProt Gene Name: | MARCKSL1�� |
| UniProt Entry Name: | MRP_HUMAN |
