Description
| Product Name: | Human MAGOH Recombinant Protein |
| Product Code: | RPPB3917 |
| Size: | 25µg |
| Species: | Human |
| Target: | MAGOH |
| Synonyms: | MAGOH1, MAGOHA, Protein mago nashi homolog, MAGOH, proliferation-associated (Drosophila). |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | MAGOH Human solution containing 20mM Tris-HCl pH-8, 2mM DTT, 0.1M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPILEHH HHHH |
Drosophila that have mutations in their MAGOH gene produce progeny with defects in germplasm assembly and germline development. MAGOH, the human homolog of Drosophila mago nashi, is necessary for embryo development. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and is induced by serum stimulation of quiescent fibroblast.
MAGOH Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 154 amino acids (1-146 a.a.) and having a molecular mass of 18.2 kDa. MAGOH protein is fused to a 8 amino acid His-Tag at C-terminus and purified by standard chromatography.
| UniProt Protein Function: | MAGOH: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Belongs to the mago nashi family. |
| UniProt Protein Details: | Protein type:RNA splicing; Spliceosome Chromosomal Location of Human Ortholog: 1p32.3 Cellular Component: nucleoplasm; nuclear speck; nucleus; cytosol Molecular Function:protein binding Biological Process: regulation of translation; transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; mRNA catabolic process, nonsense-mediated decay; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; regulation of alternative nuclear mRNA splicing, via spliceosome |
| NCBI Summary: | Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61326 |
| NCBI GenInfo Identifier: | 47117708 |
| NCBI Gene ID: | 4116 |
| NCBI Accession: | P61326.1 |
| UniProt Secondary Accession: | P61326,O35169, P50606, Q5SW69, B1ARP8, B2R5A2, |
| UniProt Related Accession: | P61326 |
| Molecular Weight: | 146 |
| NCBI Full Name: | Protein mago nashi homolog |
| NCBI Synonym Full Names: | mago-nashi homolog, proliferation-associated (Drosophila) |
| NCBI Official Symbol: | MAGOH�� |
| NCBI Official Synonym Symbols: | MAGOH1; MAGOHA�� |
| NCBI Protein Information: | protein mago nashi homolog |
| UniProt Protein Name: | Protein mago nashi homolog |
| Protein Family: | Protein mago nashi |
| UniProt Gene Name: | MAGOH�� |
| UniProt Entry Name: | MGN_HUMAN |
