Description
| Product Name: | Human LYN Recombinant Protein |
| Product Code: | RPPB3897 |
| Size: | 20µg |
| Species: | Human |
| Target: | LYN |
| Synonyms: | Tyrosine-protein kinase Lyn, Lck/Yes-related novelprotein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogenehomolog, p53Lyn, p56Lyn, JTK8, EC 2.7.10, LYN Proto-Oncogene, Src FamilyTyrosine Kinase, V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog,Lck/Yes-Related Novel Protein Tyrosine Kinase, EC 2.7.10.2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The LYN solution (1mg/ml) contains 20mM Tris-HCl buffer(pH 8.0) ,0.4M urea and10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHHSSGLVPRGSH MGSMGCIKSKGKDSLSDDGV DLKTQPVRNT ERTIYVRDPT SNKQQRPVPE SQLLPGQRFQ TKDPEEQGDI VVALYPYDGIHPDDLSFKKG EKMKVLEEHG EWWKAKSLLT KKEGFIPSNY VAKLNTLETE EWFFKDITRK DAERQLLAPGNSAGAFLIRE SETLKGSFSL SVRDFDPVHG DVIKHYKIRS LDNGGYYISP RITFPCISDM IKHYQKQADGLCRRLEKACI SPKPQKPWDK DAWEIPRESI KLVKRLGAGQ FGEVWMGYYN NSTKVAVKTL KPGTMSVQAFLEEANLMKTL QHDKLVRLYA VVTREEPIYI ITEYMAKGSL LDFLKSDEGG KVLLPKLIDF SAQIAEGMAYIERKNYIHRD LRAANVLVSE SLMCKIADFG LARVIEDNEY TAREGAKFPI KWTAPEAINF GCFTIKSDVW SFGILLYEIVTYGKIPYPGR TNADVMTALS QGYRMPRVEN CPDELYDIMK MCWKEKAEER PTFDYLQSVL DDFYTATEGQYQQQP |
Tyrosine-protein kinase Lyn (LYN) belongs to the src family of non-receptor protein tyrosine kinases, which is primarily expressed in haematopoietic tissues. LYN protein is involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. LYN protein stimulates SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN - MEKK1 - MKK7 - SAPK pathway is efficient in the induction of apoptosis by genotoxic agents.
LYN Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 535 amino acids (1-512) and having a molecular mass of 61.0kDa. LYN is fused to a 23 amino acid His-tag at N-terminus.
| UniProt Protein Function: | LYN: a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling. |
| UniProt Protein Details: | Protein type:Protein kinase, tyrosine (non-receptor); Kinase, protein; Oncoprotein; Protein kinase, TK; EC 2.7.10.2; TK group; Src family Chromosomal Location of Human Ortholog: 8q13 Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; Golgi apparatus; lipid raft; mast cell granule; mitochondrial crista; mitochondrial intermembrane space; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density Molecular Function:ATP binding; enzyme binding; gamma-tubulin binding; glycosphingolipid binding; integrin binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; platelet-derived growth factor receptor binding; protein binding; protein-tyrosine kinase activity; receptor binding; receptor signaling protein tyrosine kinase activity; SH3 domain binding; ubiquitin protein ligase binding Biological Process: adaptive immune response; axon guidance; B cell homeostasis; B cell receptor signaling pathway; blood coagulation; cellular response to extracellular stimulus; cytokine secretion; ephrin receptor signaling pathway; erythrocyte differentiation; Fc receptor mediated inhibitory signaling pathway; Fc receptor mediated stimulatory signaling pathway; histamine secretion by mast cell; immune response-regulating cell surface receptor signaling pathway; innate immune response; leukocyte migration; lipopolysaccharide-mediated signaling pathway; negative regulation of B cell proliferation; negative regulation of cell proliferation; negative regulation of immune response; negative regulation of MAP kinase activity; negative regulation of myeloid leukocyte differentiation; negative regulation of protein amino acid phosphorylation; negative regulation of toll-like receptor 2 signaling pathway; negative regulation of toll-like receptor 4 signaling pathway; neurite development; oligodendrocyte development; peptidyl-tyrosine phosphorylation; platelet activation; platelet degranulation; positive regulation of B cell receptor signaling pathway; positive regulation of cell migration; positive regulation of cell motility; positive regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tyrosine phosphorylation of STAT protein; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of B cell apoptosis; regulation of B cell receptor signaling pathway; regulation of cell adhesion mediated by integrin; regulation of cytokine production; regulation of cytokine secretion; regulation of erythrocyte differentiation; regulation of inflammatory response; regulation of mast cell activation; regulation of mast cell degranulation; regulation of protein amino acid phosphorylation; regulation of release of sequestered calcium ion into cytosol; response to amino acid stimulus; response to axon injury; response to carbohydrate stimulus; response to DNA damage stimulus; response to drug; response to hormone stimulus; response to insulin stimulus; response to organic cyclic substance; response to sterol depletion; response to toxin; signal transduction; stimulatory C-type lectin receptor signaling pathway; T cell costimulation; tolerance induction to self antigen; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction |
| NCBI Summary: | This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] |
| UniProt Code: | P07948 |
| NCBI GenInfo Identifier: | 125480 |
| NCBI Gene ID: | 4067 |
| NCBI Accession: | P07948.3 |
| UniProt Secondary Accession: | P07948,A0AVQ5, |
| UniProt Related Accession: | P07948 |
| Molecular Weight: | 56,033 Da |
| NCBI Full Name: | Tyrosine-protein kinase Lyn |
| NCBI Synonym Full Names: | LYN proto-oncogene, Src family tyrosine kinase |
| NCBI Official Symbol: | LYN�� |
| NCBI Official Synonym Symbols: | JTK8; p53Lyn; p56Lyn�� |
| NCBI Protein Information: | tyrosine-protein kinase Lyn |
| UniProt Protein Name: | Tyrosine-protein kinase Lyn |
| UniProt Synonym Protein Names: | Lck/Yes-related novel protein tyrosine kinase; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn |
| Protein Family: | Tyrosine-protein kinase |
| UniProt Gene Name: | LYN�� |
| UniProt Entry Name: | LYN_HUMAN |
