Description
| Product Name: | Human LSM4 Recombinant Protein |
| Product Code: | RPPB3885 |
| Size: | 20µg |
| Species: | Human |
| Target: | LSM4 |
| Synonyms: | U6 snRNA-associated Sm-like protein LSm4, Glycine-rich protein, GRP, LSM4, YER112W. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The LSM4 solution (0.5 mg/ml) 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRGR GGLQQQKQQK GRGMGGAGRG VFGGRGRGGI PGTGRGQPEK KPGRQAGKQ |
LSM4 (U6 snRNA-associated Sm-like protein) forms donut-shaped heptameric complexes which are involved in various steps of RNA metabolism. LSM4 facilitates RNA protein interactions and structural changes which are essential during ribosomal subunit assembly. LSM4 binds specifically to the 3'-terminal U-tract of U6 snRNA.
LSM4 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 159 amino acids (1-139 a.a.) and having a molecular mass of 17.5kDa. The LSM4 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | LSM4: Binds specifically to the 3'-terminal U-tract of U6 snRNA. Belongs to the snRNP Sm proteins family. |
| UniProt Protein Details: | Protein type:RNA-binding; Spliceosome Chromosomal Location of Human Ortholog: 19p13.11 Cellular Component: small nuclear ribonucleoprotein complex; spliceosome; cytosol Molecular Function:protein binding Biological Process: RNA splicing; gene expression; mRNA processing; mRNA catabolic process, deadenylation-dependent decay |
| NCBI Summary: | This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q9Y4Z0 |
| NCBI GenInfo Identifier: | 10720082 |
| NCBI Gene ID: | 25804 |
| NCBI Accession: | Q9Y4Z0.1 |
| UniProt Related Accession: | Q9Y4Z0 |
| Molecular Weight: | Calculated MW: 15kDaObserved MW: 15kDa |
| NCBI Full Name: | U6 snRNA-associated Sm-like protein LSm4 |
| NCBI Synonym Full Names: | LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae) |
| NCBI Official Symbol: | LSM4�� |
| NCBI Official Synonym Symbols: | GRP; YER112W�� |
| NCBI Protein Information: | U6 snRNA-associated Sm-like protein LSm4; glycine-rich protein |
| UniProt Protein Name: | U6 snRNA-associated Sm-like protein LSm4 |
| UniProt Synonym Protein Names: | Glycine-rich protein; GRP |
| Protein Family: | Sm-like protein |
| UniProt Gene Name: | LSM4�� |
| UniProt Entry Name: | LSM4_HUMAN |
