Description
| Product Name: | Human LSM1 Recombinant Protein |
| Product Code: | RPPB3881 |
| Size: | 20µg |
| Species: | Human |
| Target: | LSM1 |
| Synonyms: | U6 snRNA-associated Sm-like protein LSm1, Cancer-associated Sm-like, Small nuclear ribonuclear CaSm, LSM1, CASM, YJL124C. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The LSM1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 1mM DTT, 10% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MNYMPGTASL IEDIDKKHLV LLRDGRTLIG FLRSIDQFAN LVLHQTVERI HVGKKYGDIP RGIFVVRGEN VVLLGEIDLE KESDTPLQQV SIEEILEEQR VEQQTKLEAE KLKVQALKDR GLSIPRADTL DEY |
U6 snRNA-associated Sm-like protein LSm1 (LSM1) has a role in replication-dependent histone mRNA degradation and binds specifically to the 3''-terminal U-tract of U6 snRNA. Sm-like proteins include the Sm sequence motif, which consists of two regions separated by a linker of variable length that folds as a loop. The LSM1 protein facilitates RNA protein interactions and structural modifications which are required during ribosomal subunit assembly. The Sm-like proteins are believed to form a stable heteromer present in tri-snRNP particles, which are vital for pre-mRNA splicing. LSM1 is overexpressed in pancreatic cancer as well as in several breast cancer cell lines.
LSM1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 153 amino acids (1-133 a.a.) and having a molecular mass of 17.3kDa. The LSM1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | LSM1: Plays a role in replication-dependent histone mRNA degradation. Binds specifically to the 3'-terminal U-tract of U6 snRNA. Belongs to the snRNP Sm proteins family. |
| UniProt Protein Details: | Protein type:RNA-binding Chromosomal Location of Human Ortholog: 8p11.23 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:protein binding; RNA binding; RNA cap binding Biological Process: deadenylation-dependent decapping; RNA splicing; RNA splicing, via transesterification reactions |
| NCBI Summary: | This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Increased expression of this gene may play a role in cellular transformation and the progression of several malignancies including lung cancer, mesothelioma and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Nov 2011] |
| UniProt Code: | O15116 |
| NCBI GenInfo Identifier: | 20177943 |
| NCBI Gene ID: | 27257 |
| NCBI Accession: | O15116.1 |
| UniProt Secondary Accession: | O15116,B2R5E6, |
| UniProt Related Accession: | O15116 |
| Molecular Weight: | 15kDa |
| NCBI Full Name: | U6 snRNA-associated Sm-like protein LSm1 |
| NCBI Synonym Full Names: | LSM1 homolog, mRNA degradation associated |
| NCBI Official Symbol: | LSM1�� |
| NCBI Official Synonym Symbols: | CASM; YJL124C�� |
| NCBI Protein Information: | U6 snRNA-associated Sm-like protein LSm1 |
| UniProt Protein Name: | U6 snRNA-associated Sm-like protein LSm1 |
| UniProt Synonym Protein Names: | Cancer-associated Sm-like; Small nuclear ribonuclear CaSm |
| Protein Family: | Sm-like protein |
| UniProt Gene Name: | LSM1�� |
