Description
| Product Name: | Human LIF Recombinant Protein |
| Product Code: | RPPB0739 |
| Size: | 10µg |
| Species: | Human |
| Target: | LIF |
| Synonyms: | CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | LIF filtered (0.4 �m) and lyophilized from 0.5mg/ml solution in 20 mM Tris buffer, 20 mM NaCl and 5% w/v trehalose, pH 7.5. |
| Solubility: | It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MKHHHHHHAS PLPITPVNAT CAIRHPCHNN LMNQIRSQLA QLNGSANALF ILYYTAQGEP FPNNLDKLCG PNVTDFPPFH ANGTEKAKLV ELYRIVVYLG TSLGNITRDQ KILNPSALSL HSKLNATADI LRGLLSNVLC RLCSKYHVGH VDVTYGPDTS GKDVFQKKKL GCQLLGKYKQ IIAVLAQAF |
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence.
LIF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 23-202) containing 189 amino acids including a 9 a.a N-terminal His tag. The total molecular mass is 20.9kDa (calculated).
| UniProt Protein Function: | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. |
| NCBI Summary: | The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| UniProt Code: | P15018 |
| NCBI GenInfo Identifier: | 126279 |
| NCBI Gene ID: | 3976 |
| NCBI Accession: | P15018.1 |
| UniProt Secondary Accession: | P15018,Q52LZ2, B2RCW7, B5MC23, |
| UniProt Related Accession: | P15018 |
| Molecular Weight: | 9,563 Da |
| NCBI Full Name: | Leukemia inhibitory factor |
| NCBI Synonym Full Names: | LIF, interleukin 6 family cytokine |
| NCBI Official Symbol: | LIF�� |
| NCBI Official Synonym Symbols: | CDF; DIA; HILDA; MLPLI�� |
| NCBI Protein Information: | leukemia inhibitory factor |
| UniProt Protein Name: | Leukemia inhibitory factor |
| UniProt Synonym Protein Names: | Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN: Emfilermin |
| Protein Family: | Leukemia inhibitory factor |
| UniProt Gene Name: | LIF�� |
