Description
| Product Name: | Human LAP3 Recombinant Protein |
| Product Code: | RPPB1882 |
| Size: | 20µg |
| Species: | Human |
| Target: | LAP3 |
| Synonyms: | Leucine Aminopeptidase 3, Peptidase S, PEPS, ProlineAminopeptidase, Leucyl Aminopeptidase, ProlylAminopeptidase, EC 3.4.11.1, LAP-3, LAPEP, Epididymis Secretory Protein Li 106,Cytosol Aminopeptidase, EC 3.4.11.5, HEL-S-106, EC 3.4.11, LAP, Cytosolaminopeptidase, Leucine aminopeptidase 3, Leucyl aminopeptidase, Prolineaminopeptidase, Prolyl aminopeptidase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | LAP3 protein solution (0.5mg/ml) containing 20mMTris-HCl(pH8.5), 50% glycerol, 5mM DTT and 1mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSHMFLLPLPAAG RVVVRRLAVR RFGSRSLSTA DMTKGLVLGI YSKEKEDDVP QFTSAGENFD KLLAGKLRETLNISGPPLKA GKTRTFYGLH QDFPSVVLVG LGKKAAGIDE QENWHEGKEN IRAAVAAGCR QIQDLELSSVEVDPCGDAQA AAEGAVLGLY EYDDLKQKKK MAVSAKLYGS GDQEAWQKGV LFASGQNLAR QLMETPANEMTPTRFAEIIE KNLKSASSKT EVHIRPKSWI EEQAMGSFLS VAKGSDEPPV FLEIHYKGSP NANEPPLVFVGKGITFDSGG ISIKASANMD LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVVRAKNGKTIQV DNTDAEGRLI LADALCYAHT FNPKVILNAA TLTGAMDVAL GSGATGVFTN SSWLWNKLFEASIETGDRVW RMPLFEHYTR QVVDCQLADV NNIGKYRSAG ACTAAAFLKE FVTHPKWAHL DIAGVMTNKDEVPYLRKGMT GRPTRTLIEF LLRFSQDNA |
Leucine Aminopeptidase 3 also known as LAP3 is a member of the peptidaseM17 family. LAP3 is implicated in the processing as well as regular turnover ofintracellular proteins. LAP3 catalyzes the removal of unsubstituted N-terminalamino acids from different peptides. LAP3is responsible of releasing an N-terminal amino acid, Xaa-|-Yaa-, in which Xaais preferably Leu, but could be other amino acids including Pro although notArg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are as wellreadily hydrolyzed; however rates on arylamides are exceedingly low.
LAP3 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 539 amino acids (1-519 a.a) andhaving a molecular mass of 58.3kDa.LAP3 is fused to a 20 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | LAP3: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Belongs to the peptidase M17 family. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - glutathione; Protease; EC 3.4.11.5; Mitochondrial; EC 3.4.11.1; Amino Acid Metabolism - arginine and proline Chromosomal Location of Human Ortholog: 4p15.32 Cellular Component: cytoplasm; focal adhesion; nucleoplasm; nucleus |
| UniProt Code: | P28838 |
| NCBI GenInfo Identifier: | 124028615 |
| NCBI Gene ID: | 51056 |
| NCBI Accession: | P28838.3 |
| UniProt Secondary Accession: | P28838,Q6IAM6, Q6P0L6, Q9UQE3, B3KMQ3, |
| UniProt Related Accession: | P28838 |
| Molecular Weight: | 52,771 Da |
| NCBI Full Name: | Cytosol aminopeptidase |
| NCBI Synonym Full Names: | leucine aminopeptidase 3 |
| NCBI Official Symbol: | LAP3 |
| NCBI Official Synonym Symbols: | LAP; PEPS; LAPEP; HEL-S-106 |
| NCBI Protein Information: | cytosol aminopeptidase |
| UniProt Protein Name: | Cytosol aminopeptidase |
| UniProt Synonym Protein Names: | Leucine aminopeptidase 3; LAP-3; Leucyl aminopeptidase; Peptidase S; Proline aminopeptidase (EC:3.4.11.5); Prolyl aminopeptidase |
| Protein Family: | Leucine aminopeptidase |
| UniProt Gene Name: | LAP3 |
| UniProt Entry Name: | AMPL_HUMAN |
| Izquierdo et al. | Identification of a potent and selective LAPTc inhibitor by RapidFire-Mass Spectrometry, with antichagasic activity | PLOS Neglected Tropical Diseases 2024 | PubMed ID: 38359089 |
| Aguado et al. | Identification and Validation of Compounds Targeting Leishmania major Leucyl-Aminopeptidase M17 | Parasitology and Medicinal Chemistry 2024 | PubMed ID: 38753953 |
