Description
| Product Name: | Human KRT14 Recombinant Protein |
| Product Code: | RPPB3813 |
| Size: | 20µg |
| Species: | Human |
| Target: | KRT14 |
| Synonyms: | Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14, NFJ, CK14, EBS3, EBS4. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride. |
| Solubility: | It is recommended to reconstitute the lyophilized CK-14 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized KRT14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KRT14 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
Cytokeratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. Cytokeratin 14 is a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
Cytokeratin 14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 51,530 Dalton. The KRT14 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: cytoplasm; keratin filament; intermediate filament; cytosol; nucleus Molecular Function:protein binding; structural constituent of cytoskeleton Biological Process: epidermis development; hemidesmosome assembly; epithelial cell differentiation; response to zinc ion; response to ionizing radiation; intermediate filament bundle assembly; hair cycle; aging Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type |
| NCBI Summary: | This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P02533 |
| NCBI GenInfo Identifier: | 229463044 |
| NCBI Gene ID: | 3861 |
| NCBI Accession: | P02533.4 |
| UniProt Secondary Accession: | P02533,P08779, |
| UniProt Related Accession: | P02533 |
| Molecular Weight: | ~ 52kDa |
| NCBI Full Name: | Keratin, type I cytoskeletal 14 |
| NCBI Synonym Full Names: | keratin 14 |
| NCBI Official Symbol: | KRT14�� |
| NCBI Official Synonym Symbols: | K14; NFJ; CK14; EBS3; EBS4�� |
| NCBI Protein Information: | keratin, type I cytoskeletal 14 |
| UniProt Protein Name: | Keratin, type I cytoskeletal 14 |
| UniProt Synonym Protein Names: | Cytokeratin-14; CK-14; Keratin-14; K14 |
| Protein Family: | Putative outer capsid protein |
| UniProt Gene Name: | KRT14�� |
| UniProt Entry Name: | K1C14_HUMAN |
