Description
| Product Name: | Human KLF12 Recombinant Protein |
| Product Code: | RPPB3798 |
| Size: | 20µg |
| Species: | Human |
| Target: | KLF12 |
| Synonyms: | AP-2rep, AP2REP, HSPC122, Krueppel-like factor 12, Transcriptionalrepressor AP-2rep. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | KLF12 protein solution (0.5mg/ml) contains 20mM Tris-HClbuffer, (pH8.0) ,10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMNIHMKR KTIKNINTFE NRMLMLDGMP AVRVKTELLE SEQGSPNVHNYPDMEAVPLL LNNVKGEPPE DSLSVDHFQT QTEPVDLSIN KARTSPTAVS SSPVSMTASA SSPSSTSTSSSSSSRLASSP TVITSVSSAS SSSTVLTPGP LVASASGVGG QQFLHIIHPV PPSSPMNLQS NKLSHVHRIP VVVQSVPVVY TAVRSPGNVNNTIVVPLLED GRGHGKAQMD PRGLSPRQSK SDSDDDDLPN VTLDSVNETG STALSIARAV QEVHPSPVSRVRGNRMNNQK FPCSISPFSI ESTRRQRRSE SPDSRKRRIH RCDFEGCNKV YTKSSHLKAH RRTHTGEKPY KCTWEGCTWK FARSDELTRH YRKHTGVKPF KCADCDRSFSRSDHLALHRR RHMLV |
Krueppel-like factor 12 (KLF12) belongs to the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a particular site in the AP-2 alpha gene promoter. AP-2 alpha (Activator protein-2 alpha) is a developmentally-regulated transcription factor and vital regulator of gene expression during vertebrate development and carcinogenesis. KLF12 gene repression entails binding with a corepressor, CtBP1.
KLF12 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 425 amino acids (1-402 a.a) and having a molecular mass of 46.6kDa.KLF12 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | KLF12: Confers strong transcriptional repression to the AP-2- alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; C2H2-type zinc finger protein; DNA-bindingChromosomal Location of Human Ortholog: 13q22Cellular Component: nucleusMolecular Function: DNA binding; metal ion binding; transcription corepressor activity; transcription factor activityBiological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter |
| UniProt Protein Details: | |
| NCBI Summary: | Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. The protein encoded by this gene is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y4X4 |
| NCBI GenInfo Identifier: | 91771555 |
| NCBI Gene ID: | 11278 |
| NCBI Accession: | Q9Y4X4.2 |
| UniProt Secondary Accession: | Q9Y4X4,Q5VZM7, Q9UHZ0, A8K5T2, L0R3J4 |
| UniProt Related Accession: | Q9Y4X4 |
| Molecular Weight: | 402 |
| NCBI Full Name: | Krueppel-like factor 12 |
| NCBI Synonym Full Names: | Kruppel-like factor 12 |
| NCBI Official Symbol: | KLF12�� |
| NCBI Official Synonym Symbols: | AP2REP; AP-2rep; HSPC122�� |
| NCBI Protein Information: | Krueppel-like factor 12; AP-2 repressor; AP-2rep transcription factor; transcriptional repressor AP-2rep; KLF12 zinc finger transcriptional repressor |
| UniProt Protein Name: | Krueppel-like factor 12 |
| UniProt Synonym Protein Names: | Transcriptional repressor AP-2rep |
| Protein Family: | Krueppel-like factor |
| UniProt Gene Name: | KLF12�� |
| UniProt Entry Name: | KLF12_HUMAN |
