Description
| Product Name: | Human KIN Recombinant Protein |
| Product Code: | RPPB3784 |
| Size: | 20µg |
| Species: | Human |
| Target: | KIN |
| Synonyms: | BTCD, KIN17, KIN, Binding to curved DNA, antigenic determinant of recA protein homolog, DNA/RNA-binding protein KIN17. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The KIN solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMGKSDFL TPKAIANRIK SKGLQKLRWY CQMCQKQCRDENGFKCHCMS ESHQRQLLLA SENPQQFMDY FSEEFRNDFL ELLRRRFGTK RVHNNIVYNE YISHREHIHM NATQWETLTD FTKWLGREGL CKVDETPKGW YIQYIDRDPE TIRRQLELEKKKKQDLDDEE KTAKFIEEQV RRGLEGKEQE VPTFTELSRE NDEEKVTFNL SKGACSSSGATSSKSSTLGP SALKTIGSSA SVKRKESSQS STQSKEKKKK KSALDEIMEI EEEKKRTARTDYWLQPEIIV KIITKKLGEK YHKKKAIVKE VIDKYTAVVK MIDSGDKLKL DQTHLETVIPAPGKRILVLN GGYRGNEGTL ESINEKTFSA TIVIETGPLK GRRVEGIQYE DISKLA |
DNA/RNA-binding protein KIN17 (KIN17) is a nuclear protein which constructs intranuclear foci for the duration of proliferation and is reallocated in the nucleoplasm during the cell cycle. KIN17 is ubiqutiously expressed having the highest levels of expression in the muscle, heart and testis. SV40- altered fibroblasts overexpress KIN17, which interacts with Large T antigen and decreases T-antigen-dependent DNA replication.
KIN Human Recombinant produced in E. coli is a single polypeptide chain containing 416 amino acids (1-393) and having a molecular mass of 47.8 kDa.KIN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | KIN: Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo. Binds via its C-terminal domain to RNA in vitro. Belongs to the KIN17 family. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; DNA replication Chromosomal Location of Human Ortholog: 10p14 Cellular Component: nuclear matrix; intracellular membrane-bound organelle; cytoplasm; nucleus Molecular Function:DNA binding; RNA binding; metal ion binding; double-stranded DNA binding Biological Process: viral reproduction; DNA replication; mRNA processing; DNA repair; response to DNA damage stimulus; DNA recombination |
| NCBI Summary: | The protein encoded by this gene is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. Short-wave ultraviolet light provokes the relocalization of the protein, suggesting its participation in the cellular response to DNA damage. Originally selected based on protein-binding with RecA antibodies, the mouse protein presents a limited similarity with a functional domain of the bacterial RecA protein, a characteristic shared by this human ortholog. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| UniProt Code: | O60870 |
| NCBI GenInfo Identifier: | 74762109 |
| NCBI Gene ID: | 22944 |
| NCBI Accession: | O60870.2 |
| UniProt Related Accession: | O60870 |
| Molecular Weight: | 43kDa; 45kDa |
| NCBI Full Name: | DNA/RNA-binding protein KIN17 |
| NCBI Synonym Full Names: | Kin17 DNA and RNA binding protein |
| NCBI Official Symbol: | KIN�� |
| NCBI Official Synonym Symbols: | BTCD; Rts2; KIN17�� |
| NCBI Protein Information: | DNA/RNA-binding protein KIN17 |
| UniProt Protein Name: | DNA/RNA-binding protein KIN17 |
| UniProt Synonym Protein Names: | Binding to curved DNA; KIN, antigenic determinant of recA protein homolog |
| Protein Family: | Kinin |
| UniProt Gene Name: | KIN�� |
| UniProt Entry Name: | KIN17_HUMAN |
