Description
| Product Name: | Human IL7 Recombinant Protein |
| Product Code: | RPPB0542 |
| Size: | 10µg |
| Species: | Human |
| Target: | IL7 |
| Synonyms: | Interleukin 7, IL-7, Interleukin-7. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | IL7 protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPDCDIEGKDGKQYESVLM VSIDQLLDSM KEIGSNCLNN EFNFFKRHIC DANKEGMFLF RAARKLRQFL KMNSTGDFDLHLLKVSEGTT ILLNCTGQVK GRKPAALGEA QPTKSLEENK SLKEQKKLND LCFLKRLLQE IKTCWNKILM GTKEHHHHHHH |
IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.
IL7 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 161 amino acids (26-177 a.a.) andhaving a molecular mass of 18.4kDa (Molecular size on SDS-PAGE will appear atapproximately 18-28 kDa). IL7 is expressed with a 6 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Apoptosis; Secreted, signal peptide; Cell development/differentiation; Cytokine; Secreted; Cell cycle regulation Chromosomal Location of Human Ortholog: 8q12-q13 Cellular Component: extracellular space; extracellular region Molecular Function:interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity Biological Process: positive regulation of organ growth; humoral immune response; T cell lineage commitment; organ morphogenesis; positive regulation of T cell differentiation; cell-cell signaling; regulation of gene expression; homeostasis of number of cells within a tissue; negative regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of B cell proliferation; negative regulation of apoptosis; bone resorption |
| NCBI Summary: | The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010] |
| UniProt Code: | P13232 |
| NCBI GenInfo Identifier: | 124354 |
| NCBI Gene ID: | 3574 |
| NCBI Accession: | P13232.1 |
| UniProt Secondary Accession: | P13232,Q5FBY5, Q5FBY9, A0N0L3, |
| UniProt Related Accession: | P13232 |
| Molecular Weight: | 177 |
| NCBI Full Name: | Interleukin-7 |
| NCBI Synonym Full Names: | interleukin 7 |
| NCBI Official Symbol: | IL7�� |
| NCBI Official Synonym Symbols: | IL-7�� |
| NCBI Protein Information: | interleukin-7 |
| UniProt Protein Name: | Interleukin-7 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL7�� |
| UniProt Entry Name: | IL7_HUMAN |
