Description
| Product Name: | Human IL36A Recombinant Protein |
| Product Code: | RPPB0676 |
| Size: | 10µg |
| Species: | Human |
| Target: | IL36A |
| Synonyms: | Interleukin 36 alpha, FIL1E, IL1F6, FIL1, IL1(EPSILON), interleukin 1 family member 6 (epsilon), MGC129552, MGC129553. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in 20mM Tris-HCl, 150mM NaCl, 0.1% Tween 20, pH 8.0. |
| Solubility: | It is recommended to reconstitute the lyophilized IL36A in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IL36A Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36A should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | KIDTPQQGSI QDINHRVWVL QDQTLIAVPR KDRMSPVTIA LISCRHVETL EKDRGNPIYL GLNGLNLCLM CAKVGDQPTL QLKEKDIMDL YNQPEPVKSF LFYHSQSGRN STFESVAFPG WFIAVSSEGG CPLILTQELG KANTTDFGLT MLF |
| Biological Activity: | Fully biologically active when compared to standard. The ED50 as measured by its ability to induce IL-8 secretion in human preadipocytes is less than 10ng/ml, corresponding to a Specific Activity of 100,000 IU/mg. |
Human IL-36a belongs to the IL-1 family which includes IL-1b, IL-1a, IL-1ra, IL-18, IL-36ra (IL1F5), IL-36b (IL1F8), IL-36g (IL1F9), IL-37 (IL1F7) and IL-38 (IL-1F10). The IL-1 family members display a 12 b-strand, b-trefoil configuration, and are thought to have ascended from a mutual ancestral gene. IL-36a is an 18-22kDa, 158aa intracellular and secreted protein which holds no signal sequence, no prosegment and no potential from N-linked glycosylation sites. IL-36a is released as a reaction to LPS and the cell ATP-induced activation of the P2X7 receptor. Human IL-36a (aa 6-158) shares 57-68% aa sequence homology with mouse, rabbit, equine and bovine IL-36a and 27-57% aa sequence homology with other new IL-1 family members. IL-36a is mostly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine.
IL36A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 153 amino acids (aa 6-158) and having a molecular mass of 17.0kDa.The IL36A is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL1F6: Belongs to the IL-1 family. |
| UniProt Protein Details: | Protein type:Cytokine Chromosomal Location of Human Ortholog: 2q12-q14.1 Cellular Component: extracellular region; extracellular space Molecular Function:cytokine activity; interleukin-1 receptor binding Biological Process: cytokine and chemokine mediated signaling pathway |
| NCBI Summary: | The protein encoded by this gene is a cytokine that can activate NF-kappa-B and MAPK signaling pathways to generate an inflammatory response. The encoded protein functions primarily in skin and demonstrates increased expression in psoriasis. In addition, decreased expression of this gene has been linked to a poor prognosis in both hepatocellular carcinoma and colorectal cancer patients. [provided by RefSeq, Nov 2015] |
| UniProt Code: | Q9UHA7 |
| NCBI GenInfo Identifier: | 25008601 |
| NCBI Gene ID: | 27179 |
| NCBI Accession: | Q9UHA7.1 |
| UniProt Secondary Accession: | Q9UHA7,Q53SR7, Q5BLR4, Q7RTZ8, B2RAD9, |
| UniProt Related Accession: | Q9UHA7 |
| Molecular Weight: | 17,684 Da |
| NCBI Full Name: | Interleukin-36 alpha |
| NCBI Synonym Full Names: | interleukin 36, alpha |
| NCBI Official Symbol: | IL36A�� |
| NCBI Official Synonym Symbols: | FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)�� |
| NCBI Protein Information: | interleukin-36 alpha |
| UniProt Protein Name: | Interleukin-36 alpha |
| UniProt Synonym Protein Names: | FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6; IL-1F6 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL36A�� |
| UniProt Entry Name: | IL36A_HUMAN |
