Description
| Product Name: | Human IL 31 Recombinant Protein |
| Product Code: | RPPB0657 |
| Size: | 10µg |
| Species: | Human |
| Target: | IL 31 |
| Synonyms: | Interleukin 31, IL31, IL-31. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The IL-31 (1mg/ml) was lyophilized with 20mM Phosphate buffer, 150mM NaCl pH-7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized IL-31 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IL-31 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-31 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T |
| Biological Activity: | The ED50 was determined by its ability to activate STAT following receptor ligand interaction and found to be <5ng/ml, corresponding to a specific activity of 200,000 units. |
IL-31 produced by activated Th2-type T cells, cooperates with a heterodimeric receptor consisting of IL-31 Receptor Anatagonist and Onconstatin-M Receptor that is continuesly expressed on epithelial cells and keratinocytes. IL-31 plays a role in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. IL-31 is involved in the itching sensation and endorses the scratching behavior in NC/Nga mice with atopic dermatitis. IL-31 expression is connectd with CLA(+) T cells and contributes to the development of atopic dermatitis-induced skin inflammation and pruritus. IL-31 is a powerful inducer of proinflammatory mediators in human colonic SEMFs. IL-31 takes part as a proinflammatory cytokine derived from Th2 cells.Serum IL-31 level is higher in patients with atopic dermatitis. IL-31 is involved in a broad range of immune- & non-immune cells & possesses potential pleiotropic physiological functions, including regulating hematopoiesis & immune response, causing inflammatory bowel disease, airway hypersensitivity & dermatitis.
IL-31 human recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids (24-164 a.a.) and having a molecular mass of 15.8 kDa.
| UniProt Protein Function: | IL31: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. |
| UniProt Protein Details: | Protein type:Secreted; Cytokine; Secreted, signal peptide Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: extracellular space Molecular Function:cytokine activity Biological Process: immune system process |
| NCBI Summary: | IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q6EBC2 |
| NCBI GenInfo Identifier: | 62122911 |
| NCBI Gene ID: | 386653 |
| NCBI Accession: | NP_001014358.1 |
| UniProt Secondary Accession: | Q6EBC2,A2RUQ1, |
| UniProt Related Accession: | Q6EBC2 |
| Molecular Weight: | 18,205 Da |
| NCBI Full Name: | interleukin-31 |
| NCBI Synonym Full Names: | interleukin 31 |
| NCBI Official Symbol: | IL31�� |
| NCBI Official Synonym Symbols: | IL-31�� |
| NCBI Protein Information: | interleukin-31 |
| UniProt Protein Name: | Interleukin-31 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL31�� |
| UniProt Entry Name: | IL31_HUMAN |
