Description
| Product Name: | Human IL 20 Recombinant Protein |
| Product Code: | RPPB0630 |
| Size: | 10µg |
| Species: | Human |
| Target: | IL 20 |
| Synonyms: | IL10D, ZCYTO10, IL-20, MGC96907, Four alpha helix cytokine Zcyto10. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized carrier free. |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin -20 in sterile 18M?-cm H2O at a concentration of 0.5mg/ml or less. |
| Stability: | Lyophilized Interleukin-20 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL20 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Lys-Thr-Leu |
| Biological Activity: | The Biological Activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEKa cells).Significant STAT3 activation is observed with 100 ng/mL of recombinant human IL-20 corresponding to a Specific Activity of 10,000IU/mg. |
IL20 is a cytokine structurally related to interleukin 10 (IL10). IL20 cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.
Interleukin-20 Human Recombinant produced in E.Coli is a single, non-glycosylated homodimeric polypeptide chain containing 2 x 153 amino acids and having a total molecular mass of 35,212 Dalton. The IL-20 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL20: Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. Belongs to the IL-10 family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Cytokine; Secreted Chromosomal Location of Human Ortholog: 1q32 Cellular Component: extracellular space; extracellular region Molecular Function:interleukin-20 receptor binding; cytokine activity; interleukin-22 receptor binding Biological Process: positive regulation of osteoclast differentiation; regulation of inflammatory response; positive regulation of epidermal cell differentiation; positive regulation of JAK-STAT cascade; immune response; inflammatory response; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of keratinocyte differentiation |
| NCBI Summary: | The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NYY1 |
| NCBI GenInfo Identifier: | 38258955 |
| NCBI Gene ID: | 50604 |
| NCBI Accession: | Q9NYY1.2 |
| UniProt Secondary Accession: | Q9NYY1,Q17RB3, Q2THG6, Q96QZ6, |
| UniProt Related Accession: | Q9NYY1 |
| Molecular Weight: | 176 |
| NCBI Full Name: | Interleukin-20 |
| NCBI Synonym Full Names: | interleukin 20 |
| NCBI Official Symbol: | IL20�� |
| NCBI Official Synonym Symbols: | IL-20; IL10D; ZCYTO10�� |
| NCBI Protein Information: | interleukin-20; cytokine Zcyto10; four alpha helix cytokine |
| UniProt Protein Name: | Interleukin-20 |
| UniProt Synonym Protein Names: | Cytokine Zcyto10 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL20�� |
| UniProt Entry Name: | IL20_HUMAN |
