Description
| Product Name: | Human il 18 Recombinant Protein |
| Product Code: | RPPB0620 |
| Size: | 25µg |
| Species: | Human |
| Target: | il 18 |
| Synonyms: | IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a concentrated (1mg/ml) solution in PBS and DTT. |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18M?-cm H2O not less than 200�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Interleukin 18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Biological Activity: | The ED50 is 1.5-9 ng/mL and is measured by Interleukin 18 ability to induce IFN-? secretion by KG?1 human acute myelogenous leukemia cells in the presence of TNF-alpha. |
IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Interleukin-18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 18.2 kDa. The IL-18 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family. |
| UniProt Protein Details: | Protein type:Cytokine Chromosomal Location of Human Ortholog: 11q22.2-q22.3 Cellular Component: extracellular space; extracellular region; cytosol Molecular Function:protein binding; cytokine activity Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; positive regulation of interleukin-17 production; regulation of cell adhesion; positive regulation of natural killer cell proliferation; T-helper 1 type immune response; T-helper 2 type immune response; interleukin-2 biosynthetic process; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response |
| NCBI Summary: | The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011] |
| UniProt Code: | Q14116 |
| NCBI GenInfo Identifier: | 3219817 |
| NCBI Gene ID: | 3606 |
| NCBI Accession: | Q14116.1 |
| UniProt Secondary Accession: | Q14116,O75599, Q6FGY3, Q6WWJ7, |
| UniProt Related Accession: | Q14116 |
| Molecular Weight: | 21,896 Da |
| NCBI Full Name: | Interleukin-18 |
| NCBI Synonym Full Names: | interleukin 18 |
| NCBI Official Symbol: | IL18�� |
| NCBI Official Synonym Symbols: | IGIF; IL-18; IL-1g; IL1F4�� |
| NCBI Protein Information: | interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor) |
| UniProt Protein Name: | Interleukin-18 |
| UniProt Synonym Protein Names: | Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL18�� |
| UniProt Entry Name: | IL18_HUMAN |
