Description
| Product Name: | Human IGFBP3 Recombinant Protein |
| Product Code: | RPPB0431 |
| Size: | 25µg |
| Species: | Human |
| Target: | IGFBP3 |
| Synonyms: | GH-dependant binding protein, IBP3, BP-53, IGFBP-3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated (0.5mg/ml) solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized�IGFBP3 in sterile 20mM AcOH (acetic Acid) not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IBP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF-BP 3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK |
| Biological Activity: | The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is�< 0.2 �g/ml in the presence of 15 ng/ml of Human IGF-II, corresponding to a specific activity of 5,000,000units/mg. |
IGFBP3 is a member of the IGFBP family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with IGFALS and either IGF I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
IGFBP3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2x264 amino acids and having a molecular mass of 28806 Dalton. IGFBP-3 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. |
| UniProt Protein Details: | Protein type:Secreted; Cell development/differentiation; Secreted, signal peptide Chromosomal Location of Human Ortholog: 7p12.3 Cellular Component: extracellular region; extracellular space; insulin-like growth factor binding protein complex; nucleus Molecular Function:fibronectin binding; insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding; metal ion binding; protein binding; protein tyrosine phosphatase activator activity Biological Process: apoptosis; cellular protein metabolic process; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; negative regulation of signal transduction; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; osteoblast differentiation; positive regulation of apoptosis; positive regulation of catalytic activity; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of MAPKKK cascade; positive regulation of myoblast differentiation; protein amino acid phosphorylation; regulation of cell growth; regulation of insulin-like growth factor receptor signaling pathway |
| NCBI Summary: | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P17936 |
| NCBI GenInfo Identifier: | 146327827 |
| NCBI Gene ID: | 3486 |
| NCBI Accession: | P17936.2 |
| UniProt Secondary Accession: | P17936,Q2V509, Q6P1M6, Q9UCL4, A4D2F5, D3DVM0, |
| UniProt Related Accession: | P17936 |
| Molecular Weight: | 32,223 Da |
| NCBI Full Name: | Insulin-like growth factor-binding protein 3 |
| NCBI Synonym Full Names: | insulin like growth factor binding protein 3 |
| NCBI Official Symbol: | IGFBP3�� |
| NCBI Official Synonym Symbols: | IBP3; BP-53�� |
| NCBI Protein Information: | insulin-like growth factor-binding protein 3 |
| UniProt Protein Name: | Insulin-like growth factor-binding protein 3 |
| Protein Family: | Insulin-like growth factor-binding protein |
| UniProt Gene Name: | IGFBP3�� |
| UniProt Entry Name: | IBP3_HUMAN |
