Description
| Product Name: | Human IGFBP 1 Recombinant Protein |
| Product Code: | RPPB0430 |
| Size: | 25µg |
| Species: | Human |
| Target: | IGFBP 1 |
| Synonyms: | IBP-1, IGF-Binding Protein 1, AFBP, PP12, IGF-BP25, hIGFBP-1, IGFBP-1. |
| Source: | Mouse myeloma cell line, NS0 |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | IGFBP-1 protein was lyophilized from a 0.2�m filtered concentrated solution in PBS. |
| Solubility: | It is recommended to reconstitute the lyophilized IBP-1 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IGFBP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF-BP1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | APWQCAPCSA EKLALCPPVS ASCSEVTRSA GCGCCPMCAL PLGAACGVAT ARCARGLSCR ALPGEQQPLH ALTRGQGACV QESDASAPHA AEAGSPESPE STEITEEELL DNFHLMAPSE EDHSILWDAI STYDGSKALH VTNIKKWKEP CRIELYRVVE SLAKAQETSG EEISKFYLPN CNKNGFYHSR QCETSMDGEA GLCWCVYPWN GKRIPGSPEI RGDPNCQIYF NVQN |
| Biological Activity: | The ED50, as determined by the inhibition of rHuIGF-I-induced proliferation of human MCF-7 cells, is less than 4�g/ml. |
IGFBP1 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
IGFBP-1 Human Recombinant (26-259 a.a.) produced in NS0 is a single, glycosylated, polypeptide chain containing 234 amino acids and having a molecular mass of 25kDa. The IGFBP1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 7p12.3 Cellular Component: extracellular region; extracellular space Molecular Function:insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding Biological Process: cellular protein metabolic process; insulin receptor signaling pathway; positive regulation of cell growth; regulation of insulin-like growth factor receptor signaling pathway; signal transduction; tissue regeneration; unfolded protein response |
| NCBI Summary: | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08833 |
| NCBI GenInfo Identifier: | 124055 |
| NCBI Gene ID: | 3484 |
| NCBI Accession: | P08833.1 |
| UniProt Secondary Accession: | P08833,Q8IYP5, A4D2F4, D3DVL9, |
| UniProt Related Accession: | P08833 |
| Molecular Weight: | 27,904 Da |
| NCBI Full Name: | Insulin-like growth factor-binding protein 1 |
| NCBI Synonym Full Names: | insulin like growth factor binding protein 1 |
| NCBI Official Symbol: | IGFBP1�� |
| NCBI Official Synonym Symbols: | AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1�� |
| NCBI Protein Information: | insulin-like growth factor-binding protein 1 |
| UniProt Protein Name: | Insulin-like growth factor-binding protein 1 |
| UniProt Synonym Protein Names: | Placental protein 12; PP12 |
| Protein Family: | Insulin-like growth factor-binding protein |
| UniProt Gene Name: | IGFBP1�� |
| UniProt Entry Name: | IBP1_HUMAN |
