Description
| Product Name: | Human IER3 Recombinant Protein |
| Product Code: | RPPB3735 |
| Size: | 10µg |
| Species: | Human |
| Target: | IER3 |
| Synonyms: | immediate early response 3, DIF-2, DIF2, GLY96, IEX-1,IEX-1L, IEX1, PRG1, Protein DIF-2, Immediate early protein GLY96, PACAP-responsive gene 1protein, Differentiation-dependent gene 2 protein, IER3. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | IER3 protein solution (0.25mg/ml) contains 20mM Tris-HClbuffer, (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMCHSRSC HPTMTILQAP TPAPSTIPGP RRGSGPEIFT FDPLPEPAAAPAGRPSASRG HRKRSRRVLY PRVVRRQLPV EEPNP |
Immediate Early Response 3 (IER3) functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. IER3 has a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. In addition, IER3 serves as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, IER3 provides pancreatic ductal adenocarcinoma with significant resistance to cell stress, such as starvation.
IER3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 105 amino acids (1-82 a.a.) and having a molecular mass of 11.4kDa.IER3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IEX-1: May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A- PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. Belongs to the IER3 family. |
| UniProt Protein Details: | Protein type:Apoptosis; Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cytosol; nucleus Molecular Function:protein binding Biological Process: anatomical structure morphogenesis; apoptosis; regulation of phosphoinositide 3-kinase cascade |
| NCBI Summary: | This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P46695 |
| NCBI GenInfo Identifier: | 317373569 |
| NCBI Gene ID: | 8870 |
| NCBI Accession: | P46695.4 |
| UniProt Secondary Accession: | P46695,Q5SU30, Q92691, Q93044, |
| UniProt Related Accession: | P46695 |
| Molecular Weight: | 16,903 Da |
| NCBI Full Name: | Radiation-inducible immediate-early gene IEX-1 |
| NCBI Synonym Full Names: | immediate early response 3 |
| NCBI Official Symbol: | IER3�� |
| NCBI Official Synonym Symbols: | DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L�� |
| NCBI Protein Information: | radiation-inducible immediate-early gene IEX-1 |
| UniProt Protein Name: | Radiation-inducible immediate-early gene IEX-1 |
| UniProt Synonym Protein Names: | Differentiation-dependent gene 2 protein; Protein DIF-2; Immediate early protein GLY96; Immediate early response 3 protein; PACAP-responsive gene 1 protein; Protein PRG1 |
| Protein Family: | Radiation-inducible immediate-early gene |
| UniProt Gene Name: | IER3�� |
| UniProt Entry Name: | IEX1_HUMAN |
