Description
| Product Name: | Human HSPH1 Recombinant Protein |
| Product Code: | RPPB5233 |
| Size: | 25µg |
| Species: | Human |
| Target: | HSPH1 |
| Synonyms: | HSPH1, Heat Shock protein 105kDa, 110kDa protein 1, Heat shock 110 kDa protein, HSP110, HSP105A, Antigen NY-CO-25, HSP105, HSP105A, HSP105B, KIAA0201, NY-CO-25, DKFZp686M05240. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The HSP105 protein solution contains 20mM Tris-HCl, pH-8 and 50mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSVV GLDVGSQSCY IAVARAGGIE TIANEFSDRC TPSVISFGSK NRTIGVAAKN QQITHANNTV SNFKRFHGRA FNDPFIQKEK ENLSYDLVPL KNGGVGIKVM YMGEEHLFSV EQITAMLLTK LKETAENSLK KPVTDCVISV PSFFTDAERR SVLDAAQIVG LNCLRLMNDM TAVALNYGIY KQDLPSLDEK PRIVVFVDMG HSAFQVSACA FNKGKLKVLG TAFDPFLGGK NFDEKLVEHF CAEFKTKYKL DAKSKIRALL RLYQECEKLK KLMSSNSTDL PLNIECFMND KDVSGKMNRS QFEELCAELL QKIEVPLYSL LEQTHLKVED VSAVEIVGGA TRIPAVKERI AKFFGKDIST TLNADEAVAR GCALQCAILS PAFKVREFSV TDAVPFPISL IWNHDSEDTE GVHEVFSRNH AAPFSKVLTF LRRGPFELEA FYSDPQGVPY PEAKIGRFVV QNVSAQKDGE KSRVKVKVRV NTHGIFTIST ASMVEKVPTE ENEMSSEADM ECLNQRPPEN PDTDKNVQQD NSEAGTQPQV QTDAQQTSQS PPSPELTSEE NKIPDADKAN EKKVDQPPEA KKPKIKVVNV ELPIEANLVW QLGKDLLNMY IETEGKMIMQ DKLEKERNDA KNAVEEYVYE FRDKLCGPYE KFICEQDHQN FLRLLTETED WLYEEGEDQA KQAYVDKLEE LMKIGTPVKV RFQEAEERPK MFEELGQRLQ HYAKIAADFR NKDEKYNHID ESEMKKVEKS VNEVMEWMNN VMNAQAKKSL DQDPVVRAQE IKTKIKELNN TCEPVVTQPK PKIESPKLER TPNGPNIDKK EEDLEDKNNF GAEPPHQNGE CYPNEKNSVN MDLD |
HSPH1 analysis is used as an indicator and as a diagnostic aid in problematic lesions. HSPH1 chaperones the responses to endoplasmic reticulum (ER) stress during its interactions with GRP78 and GSK3, and without HSP105 cell death following ER stress proceeds by a non-caspase-3-dependent process. HSPH1 is highly expressed in a variety of human tumors.HSPH1 is a mammalian member of the HSP105/110 family, a diverged subgroup of the HSP70 family. HSP105 has 2 isoforms, alpha and beta. Hsp105a associates with Hsp70/Hsc70 as complexes in vivo and regulates the chaperone activity of Hsp70/Hsc70 negatively in vitro and in vivo.
Recombinant HSPH1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 894 amino acids and having a molecular mass of 100.9kDa. HSP105 Alpha is fused with a 36 a.a.�His tag and purified by conventional chromatography techniques.
| UniProt Protein Function: | HSP105: a molecular chaperone of the heat shock protein 70 family. Has ATPase activity. Expressed at especially high levels in mammalian brain and has been shown to suppress apoptosis in neuronal cells and to prevent the aggregation of proteins following heat shock. Known to interact with a variety of proteins including alpha-tubulin and the androgen receptor. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Heat shock protein; Chaperone Chromosomal Location of Human Ortholog: 13q12.3 Cellular Component: nucleoplasm; microtubule; cytoplasm; extracellular region; cytosol Molecular Function:protein binding; ATP binding; alpha-tubulin binding Biological Process: receptor-mediated endocytosis; positive regulation of NK T cell activation; chaperone cofactor-dependent protein folding; positive regulation of MHC class I biosynthetic process; response to unfolded protein |
| UniProt Code: | Q92598 |
| NCBI GenInfo Identifier: | 2495344 |
| NCBI Gene ID: | 10808 |
| NCBI Accession: | Q92598.1 |
| UniProt Secondary Accession: | Q92598,O95739, Q5TBM6, Q5TBM7, Q5TBM8, Q9UPC4, B4DYH1 |
| UniProt Related Accession: | Q92598 |
| Molecular Weight: | 97,460 Da |
| NCBI Full Name: | Heat shock protein 105 kDa |
| NCBI Synonym Full Names: | heat shock 105kDa/110kDa protein 1 |
| NCBI Official Symbol: | HSPH1�� |
| NCBI Official Synonym Symbols: | HSP105; HSP105A; HSP105B; NY-CO-25�� |
| NCBI Protein Information: | heat shock protein 105 kDa; antigen NY-CO-25; heat shock 105kD beta; heat shock 105kD alpha |
| UniProt Protein Name: | Heat shock protein 105 kDa |
| UniProt Synonym Protein Names: | Antigen NY-CO-25; Heat shock 110 kDa protein |
| Protein Family: | Heat shock protein |
| UniProt Gene Name: | HSPH1�� |
| UniProt Entry Name: | HS105_HUMAN |
