Description
| Product Name: | Human HSP27 Recombinant Protein |
| Product Code: | RPPB5235 |
| Size: | 50µg |
| Species: | Human |
| Target: | HSP27 |
| Synonyms: | HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The HSP27 protein solution contains 20mM Hepes pH-7.5, 1mM DTT and 100mM KCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK |
HSP27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38).
Recombinant Human HSP-27 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 205 amino acids and having a molecular mass of 22.7 kDa. Hsp27 was over-expressed in E. coli and purified by conventional chromatography.
| UniProt Protein Function: | HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock. |
| UniProt Protein Details: | Protein type:Chaperone; Motility/polarity/chemotaxis; Heat shock protein Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; Z disc; nucleus; cytosol Molecular Function:identical protein binding; protein kinase C inhibitor activity; protein binding; ubiquitin binding; protein kinase C binding; protein kinase binding Biological Process: positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; retinal homeostasis; positive regulation of angiogenesis; response to virus; positive regulation of blood vessel endothelial cell migration; negative regulation of protein kinase activity; gene expression; vascular endothelial growth factor receptor signaling pathway; cell motility; regulation of I-kappaB kinase/NF-kappaB cascade; response to unfolded protein; negative regulation of apoptosis; regulation of translational initiation Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f |
| NCBI Summary: | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008] |
| UniProt Code: | P04792 |
| NCBI GenInfo Identifier: | 19855073 |
| NCBI Gene ID: | 3315 |
| NCBI Accession: | P04792.2 |
| UniProt Secondary Accession: | P04792,Q6FI47, Q96C20, Q96EI7, Q9UC31, Q9UC34, Q9UC35 Q9UC36, B2R4N8, |
| UniProt Related Accession: | P04792 |
| Molecular Weight: | 205 |
| NCBI Full Name: | Heat shock protein beta-1 |
| NCBI Synonym Full Names: | heat shock 27kDa protein 1 |
| NCBI Official Symbol: | HSPB1�� |
| NCBI Official Synonym Symbols: | CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102�� |
| NCBI Protein Information: | heat shock protein beta-1; HSP 27; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein; epididymis secretory protein Li 102 |
| UniProt Protein Name: | Heat shock protein beta-1 |
| UniProt Synonym Protein Names: | 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27 |
| Protein Family: | Heat shock protein |
| UniProt Gene Name: | HSPB1�� |
| UniProt Entry Name: | HSPB1_HUMAN |
