Description
| Product Name: | Human HMGN1 Recombinant Protein |
| Product Code: | RPPB3701 |
| Size: | 10µg |
| Species: | Human |
| Target: | HMGN1 |
| Synonyms: | HMG14, GC104230, High-Mobility Group Nucleosome Binding Domain 1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | HMGN1 protein solution (0.5mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl, 0.1mM PMSF & 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MPKRKVSSAE GAAKEEPKRR SARLSAKPPA KVEAKPKKAA AKDKSSDKKV QTKGKRGAKG KQAEVANQET KEDLPAENGE TKTEESPASD EAGEKEAKSD LEHHHHHH |
HMGN1 binds to the inner side of the nucleosomal DNA therefore changing the interaction between the DNA and the histone octamer. HMGN1 participates in the development which maintains transcribable genes in an exceptional chromatin conformation. HMGN1 inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.
HMGN1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 108 amino acids (1-100 a.a.) and having a molecular mass of 11.7 kDa. HMGN1 protein is fused to an 8 amino acid His-Tag at C-terminus and purified by standard chromatography.
| UniProt Protein Function: | HMGN1: a nonhistone chromosomal protein of the HMG-14/HMG-17 protein family. Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. |
| UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 21q22.2 Cellular Component: nucleoplasm; nucleus Molecular Function:DNA binding Biological Process: positive regulation of RNA elongation; transcription-coupled nucleotide-excision repair |
| NCBI Summary: | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P05114 |
| NCBI GenInfo Identifier: | 123101 |
| NCBI Gene ID: | 3150 |
| NCBI Accession: | P05114.3 |
| UniProt Secondary Accession: | P05114,Q3KQR8, |
| UniProt Related Accession: | P05114 |
| Molecular Weight: | 10,659 Da |
| NCBI Full Name: | Non-histone chromosomal protein HMG-14 |
| NCBI Synonym Full Names: | high mobility group nucleosome binding domain 1 |
| NCBI Official Symbol: | HMGN1�� |
| NCBI Official Synonym Symbols: | HMG14�� |
| NCBI Protein Information: | non-histone chromosomal protein HMG-14 |
| UniProt Protein Name: | Non-histone chromosomal protein HMG-14 |
| UniProt Synonym Protein Names: | High mobility group nucleosome-binding domain-containing protein 1 |
| Protein Family: | Non-histone chromosomal protein |
| UniProt Gene Name: | HMGN1�� |
