Description
| Product Name: | Human HLA-DOA Recombinant Protein |
| Product Code: | RPPB3687 |
| Size: | 20µg |
| Species: | Human |
| Target: | HLA-DOA |
| Synonyms: | HLA-DNA, HLA-DZA, HLADZ, HLA class II histocompatibility antigen, DO alpha chain, MHC DN-alpha, MHC DZ alpha, MHC class II antigen DOA, HLA-DOA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The HLA-DOA solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSTKADHMG SYGPAFYQSY GASGQFTHEF DEEQLFSVDL KKSEAVWRLP EFGDFARFDP QGGLAGIAAI KAHLDILVER SNRSRAINVP PRVTVLPKSR VELGQPNILI CIVDNIFPPV INITWLRNGQ TVTEGVAQTS FYSQPDHLFR KFHYLPFVPS AEDVYDCQVE HWGLDAPLLR HWELQVPIPP PDAME |
Major Histocompatibility Complex Class II DO Alpha (HLA-DOA) is a significant modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. HLA-DOA forms a heterodimer along with HLA-DOB. The heterodimer is located in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. HLA-DOA exhibits very little sequence variation, particularly at the protein level.
HLA-DOA Human Recombinant produced in E. coli is a single polypeptide chain containing 215 amino acids (26-217) and having a molecular mass of 24.1kDa. HLA-DOA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HLA-DOA: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: endosome membrane; integral to membrane; lysosomal membrane; MHC class II protein complex; plasma membrane Molecular Function:MHC class II receptor activity; protein binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; negative regulation of antigen processing and presentation of peptide antigen via MHC class II; regulation of T cell differentiation; signal transduction |
| NCBI Summary: | HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P06340 |
| NCBI GenInfo Identifier: | 122210 |
| NCBI Gene ID: | 3111 |
| NCBI Accession: | P06340.1 |
| UniProt Secondary Accession: | P06340,Q58HU0, Q58HU1, Q5STC7, Q9TQC6, Q9TQC7, Q9TQC8 Q9TQC9, Q9TQD0, Q9TQD1, Q9TQD2, Q9TQD3, |
| UniProt Related Accession: | P06340 |
| Molecular Weight: | 27,599 Da |
| NCBI Full Name: | HLA class II histocompatibility antigen, DO alpha chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class II, DO alpha |
| NCBI Official Symbol: | HLA-DOA�� |
| NCBI Official Synonym Symbols: | HLADZ; HLA-DNA; HLA-DZA�� |
| NCBI Protein Information: | HLA class II histocompatibility antigen, DO alpha chain |
| UniProt Protein Name: | HLA class II histocompatibility antigen, DO alpha chain |
| UniProt Synonym Protein Names: | MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOA |
| Protein Family: | HLA class II histocompatibility antigen |
| UniProt Gene Name: | HLA-DOA�� |
| UniProt Entry Name: | DOA_HUMAN |
