Description
| Product Name: | Human Haptoglobin Recombinant Protein |
| Product Code: | RPPB3658 |
| Size: | 10µg |
| Species: | Human |
| Target: | Haptoglobin |
| Synonyms: | Haptoglobin isoform 2, HP, BP, HP2ALPHA2, HPA1S. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | Haptoglobin protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADLVDSGNDV TDIADDGCPK PPEIAHGYVE HSVRYQCKNY YKLRTEGDGV YTLNNEKQWI NKAVGDKLPE CEAVCGKPKN PANPVQRILG GHLDAKGSFP WQAKMVSHHN LTTGATLINE QWLLTTAKNL FLNHSENATA KDIAPTLTLY VGKKQLVEIE KVVLHPNYSQ VDIGLIKLKQ KVSVNERVMP ICLPSKDYAE VGRVGYVSGW GRNANFKFTD HLKYVMLPVA DQDQCIRHYE GSTVPEKKTP KSPVGVQPIL NEHTFCAGMS KYQEDTCYGD AGSAFAVHDL EEDTWYATGI LSFDKSCAVA EYGVYVKVTS IQDWVQKTIA ENHHHHHH |
Haptoglobin or HP is encoded by the HP gene in humans. Haptoglobin binds free hemoglobin (which is released from erythrocytes) in the blood plasma, therefore inhibits the Hb oxidative activity. The complex that contains Haptoglobin and hemoglobin will then be eliminated mostly through the spleen.�
Haptoglobin produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (19-347 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 338 amino acids and having a molecular mass of 37.7kDa. Haptoglobin shows multiple bands between 40-57kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.�
| UniProt Protein Function: | HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 16q22.2 Cellular Component: extracellular space; extracellular region Molecular Function:antioxidant activity; protein binding; hemoglobin binding; catalytic activity Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response Disease: Anhaptoglobinemia |
| NCBI Summary: | This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014] |
| UniProt Code: | P00738 |
| NCBI GenInfo Identifier: | 123508 |
| NCBI Gene ID: | 3240 |
| NCBI Accession: | P00738.1 |
| UniProt Secondary Accession: | P00738,P00737, Q0VAC4, Q0VAC5, Q2PP15, Q3B7J0, Q6LBY9 Q9UC67, B0AZL5, |
| UniProt Related Accession: | P00738 |
| Molecular Weight: | |
| NCBI Full Name: | Haptoglobin |
| NCBI Synonym Full Names: | haptoglobin |
| NCBI Official Symbol: | HP�� |
| NCBI Official Synonym Symbols: | BP; HPA1S; HP2ALPHA2�� |
| NCBI Protein Information: | haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide |
| UniProt Protein Name: | Haptoglobin |
| UniProt Synonym Protein Names: | ZonulinCleaved into the following 2 chains:Haptoglobin alpha chain; Haptoglobin beta chain |
| Protein Family: | Haptoglobin |
| UniProt Gene Name: | HP�� |
| UniProt Entry Name: | HPT_HUMAN |
