Description
| Product Name: | Human GTF2F2 Recombinant Protein |
| Product Code: | RPPB3648 |
| Size: | 20µg |
| Species: | Human |
| Target: | GTF2F2 |
| Synonyms: | General transcription factor IIF subunit 2, ATP-dependent helicase GTF2F2, General transcription factor IIF 30 kDa subunit, Transcription initiation factor IIF subunit beta, TFIIF-beta, Transcription initiation factor RAP30, GTF2F2, RAP30, BTF4, TF2F2, TFIIF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | GTF2F2 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.2M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS SDKLSLEGIV VQRAECRPAA SENYMRLKRL QIEESSKPVR LSQQLDKVVT TNYKPVANHQYNIEYERKKK EDGKRARADK QHVLDMLFSA FEKHQYYNLK DLVDITKQPV VYLKEILKEI GVQNVKGIHK NTWELKPEYR HYQGEEKSD |
General Transcription Factor IIF Polypeptide 2 (GTF2F2) is a general transcription initiation factor which binds to RNA polymerase II and helps engage it in the initiation complex in collaboration with TFIIB. GTF2F2 promotes transcription elongation. GTF2F2 shows ATP-dependent DNA-helicase activity.
GTF2F2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 269 amino acids (1-249 a.a) and having a molecular mass of 30.5kDa (Molecular weight on SDS-PAGE will appear higher).GTF2F2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GTF2F2: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity. Belongs to the TFIIF beta subunit family. |
| UniProt Protein Details: | Protein type:Helicase; EC 3.6.4.12; Nuclear receptor co-regulator; Transcription initiation complex Chromosomal Location of Human Ortholog: 13q14 Cellular Component: nucleoplasm; microtubule cytoskeleton; transcription factor TFIIF complex; nucleus Molecular Function:protein binding; DNA binding; ATP-dependent helicase activity; ATP binding Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; regulation of transcription, DNA-dependent; positive regulation of viral transcription; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression |
| UniProt Code: | P13984 |
| NCBI GenInfo Identifier: | 464519 |
| NCBI Gene ID: | 2963 |
| NCBI Accession: | P13984.2 |
| UniProt Secondary Accession: | P13984,Q5W0H3, A6NNS5, |
| UniProt Related Accession: | P13984 |
| Molecular Weight: | 249 |
| NCBI Full Name: | General transcription factor IIF subunit 2 |
| NCBI Synonym Full Names: | general transcription factor IIF, polypeptide 2, 30kDa |
| NCBI Official Symbol: | GTF2F2�� |
| NCBI Official Synonym Symbols: | BTF4; RAP30; TF2F2; TFIIF�� |
| NCBI Protein Information: | general transcription factor IIF subunit 2; TFIIF-beta; ATP-dependent helicase GTF2F2; transcription initiation factor RAP30; general transcription factor IIF 30 kDa subunit; transcription initiation factor IIF subunit beta |
| UniProt Protein Name: | General transcription factor IIF subunit 2 |
| UniProt Synonym Protein Names: | ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; Transcription initiation factor IIF subunit beta; TFIIF-beta; Transcription initiation factor RAP30 |
| Protein Family: | General transcription factor |
| UniProt Gene Name: | GTF2F2�� |
| UniProt Entry Name: | T2FB_HUMAN |
