The Human GPC3 (Glypican-3) ELISA Kit is specifically designed for the accurate measurement of Glypican-3 levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and consistent results for various research applications.Glypican-3 is a cell surface protein that plays a critical role in cell growth, proliferation, and migration. It is known to be overexpressed in certain types of cancer, such as hepatocellular carcinoma, making it a valuable biomarker for studying cancer progression and potential therapeutic interventions.
This ELISA kit is a valuable tool for researchers studying Glypican-3 and its implications in cancer biology and other diseases. Its reliable performance and ease of use make it an essential component of any laboratory conducting research in this field.
Product Name:
Human GPC3 (Glypican 3) ELISA Kit
SKU:
HUES02702
Target:
Human GPC3 (Glypican 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GPC3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GPC3. You can calculate the concentration of Human GPC3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
97-114
87-100
Average (%)
95
104
92
1:4
Range (%)
88-102
84-94
89-100
Average (%)
94
89
94
1:8
Range (%)
93-107
84-97
86-98
Average (%)
99
89
91
1:16
Range (%)
93-107
81-94
87-98
Average (%)
98
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.39
3.92
0.5
1.46
3.64
Standard deviation
0.03
0.07
0.19
0.03
0.08
0.14
C V (%)
5.56
5.04
4.85
6.0
5.48
3.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GPC3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPC3 in samples. No significant cross-reactivity or interference between Human GPC3 and analogues was observed.
