Description
| Product Name: | Human GOT2 Recombinant Protein |
| Product Code: | RPPB1726 |
| Size: | 10µg |
| Species: | Human |
| Target: | GOT2 |
| Synonyms: | EC 2.6.1.1, Aspartate aminotransferase 1, Transaminase A, GIG18. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The GOT2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYVLPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQRFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKRNLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVESQLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSSNVGYLAHAIH QVTK |
| Biological Activity: | Specific activity is > 60 units/mg, and is defined asthe amount of enzyme that convert 1umole of ?-ketoglutarate to L-Glutamate per minuteat pH 8.0 at 25C. |
GOT2 is a pyridoxal phosphate-dependent enzyme which is found in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2. GOT2 is invloved in amino acid metabolism and the urea and tricarboxylic acid cycles. The 2 enzymes are homodimeric and demonstrate close homology.
GOT2 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (30-430 a.a.) and having a molecular mass of 47kDa. The GOT2 fused to a 23 amino acid his tag at N-terminus and is purified by proprietary chromatographic techniques.
| UniProt Code: | P00505 |
| NCBI GenInfo Identifier: | 73486658 |
| NCBI Gene ID: | 2806 |
| NCBI Accession: | NP_002071.2 |
| UniProt Secondary Accession: | P00505,Q53FL3, Q9BWA3, |
| UniProt Related Accession: | P00505 |
| Molecular Weight: | 430 |
| NCBI Full Name: | aspartate aminotransferase, mitochondrial |
| NCBI Synonym Full Names: | glutamic-oxaloacetic transaminase 2, mitochondrial |
| NCBI Official Symbol: | GOT2�� |
| NCBI Official Synonym Symbols: | KAT4; KATIV; mitAAT�� |
| NCBI Protein Information: | aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; mAspAT; transaminase A; aspartate transaminase 2; fatty acid-binding protein; aspartate aminotransferase 2; kynurenine aminotransferase 4; kynurenine aminotransferase IV; glutamate oxaloacetate tr |
| UniProt Protein Name: | Aspartate aminotransferase, mitochondrial |
| UniProt Synonym Protein Names: | Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A |
| UniProt Gene Name: | GOT2�� |
| UniProt Entry Name: | AATM_HUMAN |
