Description
| Product Name: | Human GFER Recombinant Protein |
| Product Code: | RPPB3589 |
| Size: | 10µg |
| Species: | Human |
| Target: | GFER |
| Synonyms: | FAD-linked sulfhydryl oxidase ALR, Augmenter of liver regeneration, Hepatopoietin, GFER, ALR, HERV1, HPO, ALR, HSS, ERV1, HPO1, HPO2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | GFER protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAAPGE RGRFHGGNLF FLPGGARSEM MDDLATDARG RGAGRRDAAA SASTPAQAPT SDSPVAEDAS RRRPCRACVD FKTWMRTQQK RDTKFREDCP PDREELGRHS WAVLHTLAAY YPDLPTPEQQ QDMAQFIHLF SKFYPCEECA EDLRKRLCRN HPDTRTRACF TQWLCHLHNE VNRKLGKPDF DCSKVDERWR DGWKDGSCD |
FAD-linked sulfhydryl oxidase ALR (GFER) is a member of the Erv1/ALR family of proteins, which is found in higher and lower eukaryotes. GFER is a hepatotrophic growth factor and flavin-linked sulfhydryl oxidase expressed in a variety of tissues. Moreover, GFER induces the expression of S-adenosylmethionine decarboxyl-ase and ornithine decarboxylases (ODC), which each have a central role in the synthesis of polyamines. The hepatotrophic factor designated augmenter of liver regeneration (ALR) is assumed to be one of the factors responsible for the exceptional regenerative capacity of mammalian liver. The GFER gene is located on chromosome 16 in the interval containing the locus for polycystic kidney disease (PKD1).
GFER Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 229 amino acids (1-205 a.a) and having a molecular mass of 26kDa.GFER is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GFER: Isoform 1: FAD-dependent sulfhydryl oxidase. Within the mitochondrial intermembrane space, participates in a chain of disulfide exchange reactions with MIA40, that generate disulfide bonds in a number of resident proteins with twin Cx3C and Cx9C motifs. Defects in GFER are a cause of mitochondrial progressive myopathy with congenital cataract hearing loss and developmental delay (MPMCHD); also called combined mitochondrial complex deficiency. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; EC 1.8.3.2; Oxidoreductase Chromosomal Location of Human Ortholog: 16p13.3-p13.12 Cellular Component: cytoplasm; mitochondrion Molecular Function:FAD binding; protein binding; protein disulfide oxidoreductase activity Disease: Myopathy, Mitochondrial Progressive, With Congenital Cataract, Hearing Loss, And Developmental Delay |
| NCBI Summary: | The hepatotrophic factor designated augmenter of liver regeneration (ALR) is thought to be one of the factors responsible for the extraordinary regenerative capacity of mammalian liver. It has also been called hepatic regenerative stimulation substance (HSS). The gene resides on chromosome 16 in the interval containing the locus for polycystic kidney disease (PKD1). The putative gene product is 42% similar to the scERV1 protein of yeast. The yeast scERV1 gene had been found to be essential for oxidative phosphorylation, the maintenance of mitochondrial genomes, and the cell division cycle. The human gene is both the structural and functional homolog of the yeast scERV1 gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P55789 |
| NCBI GenInfo Identifier: | 218511915 |
| NCBI Gene ID: | 2671 |
| NCBI Accession: | P55789.2 |
| UniProt Secondary Accession: | P55789,Q53YM6, Q8TAH6, Q9H290, Q9UK40, |
| UniProt Related Accession: | P55789 |
| Molecular Weight: | 15,029 Da |
| NCBI Full Name: | FAD-linked sulfhydryl oxidase ALR |
| NCBI Synonym Full Names: | growth factor, augmenter of liver regeneration |
| NCBI Official Symbol: | GFER�� |
| NCBI Official Synonym Symbols: | ALR; HPO; HSS; ERV1; HPO1; HPO2; HERV1�� |
| NCBI Protein Information: | FAD-linked sulfhydryl oxidase ALR |
| UniProt Protein Name: | FAD-linked sulfhydryl oxidase ALR |
| UniProt Synonym Protein Names: | Augmenter of liver regeneration; hERV1; Hepatopoietin |
| Protein Family: | FAD-linked sulfhydryl oxidase |
| UniProt Gene Name: | GFER�� |
| UniProt Entry Name: | ALR_HUMAN |
