Description
| Product Name: | Human GATC Recombinant Protein |
| Product Code: | RPPB1675 |
| Size: | 10µg |
| Species: | Human |
| Target: | GATC |
| Synonyms: | Glutamyl-TRNA(Gln) Amidotransferase Subunit C, Glu-AdTSubunit C, 15E1.2, Glutamyl-TRNA(Gln) Amidotransferase Subunit C Homolog(Bacterial), Glutamyl-TRNA(Gln) Amidotransferase Subunit C Mitochondrial, Glutamyl-TRNA(Gln)Amidotransferase Subunit C Homolog, EC 6.3.5, Protein 15E1.2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | GATC protein solution (0.25mg/ml) containing 20mMTris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVDFGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVAPPGNISLPKL DEQEPFPHS |
Glutamyl-TRNAAmidotransferase, Subunit C also known as GATC allows the formation of properlycharged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) inthe mitochondria. The reaction occurs in the attendance of glutamine and ATP throughan activated gamma-phospho-Glu-tRNA(Gln). In addition, GATC is subunit of theheterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112)and C (GATC) subunits.
GATC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 159 amino acids (1-136 a.a) and having a molecular mass of 17.5kDa (Molecular size on SDS-PAGE will appear higher).GATC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GATC: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). Belongs to the GatC family. |
| UniProt Protein Details: | Protein type:Mitochondrial; EC 6.3.5.- Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: mitochondrion Molecular Function:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; protein binding Biological Process: mitochondrial translation |
| UniProt Code: | O43716 |
| NCBI GenInfo Identifier: | 76828060 |
| NCBI Gene ID: | 283459 |
| NCBI Accession: | BC107145 |
| UniProt Secondary Accession: | O43716,Q3B824, Q3KNR8, B3KSU7, |
| UniProt Related Accession: | O43716 |
| Molecular Weight: | 15,086 Da |
| NCBI Full Name: | Homo sapiens glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial), mRNA |
| NCBI Synonym Full Names: | glutamyl-tRNA amidotransferase subunit C |
| NCBI Official Symbol: | GATC�� |
| NCBI Official Synonym Symbols: | 15E1.2�� |
| NCBI Protein Information: | glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial |
| UniProt Protein Name: | Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial |
| UniProt Synonym Protein Names: | Protein 15E1.2 |
| Protein Family: | PTS system galactitol-specific EIIC component |
| UniProt Gene Name: | GATC�� |
| UniProt Entry Name: | GATC_HUMAN |
