Description
| Product Name: | Human Free Triiodothyronine ELISA Kit |
| SKU: | HDES0100 |
| Size: | 96T |
| Target: | FT3 |
| Detection Method: | Competition ELISA |
| Result Type: | Quantitative: Sensitivity 98.3%, Specificity 99.9% |
| Sample Type: | Serum |
| Assay Time: | 1 h 45 min |
| Detection Wavelength: | 450/630nm |
| Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Competition-ELISA as the method to detect the Free Triiodothyronine (FT3) in human serum. The ELISA Microtiter plate provided in this kit has been pre-coated with anti- T3 synthetic analogue antibody. Add samples, biotin labeled T3 synthetic analogue and HRP-streptavidin conjugate in sequence. If FT3 exist in sample will competition with biotin labeled T3 synthetic analogue, finally form a anti-T3 synthetic analogue antibody, biotin labeled T3 synthetic analogue and HRP-streptavidin conjugate complex. Free components are washed away. The substrate reagent is added to initiate the color developing reaction. The presence of FT3 can be determined according to the absorbance value by using a microplate reader with 450 nm (630 nm) wavelength.
